Invitrogen™ TNF alpha Monoclonal Antibody (E10041)

Catalog No.	Quantity
PIM3TNFAI	500 μg

Catalog No. PIM3TNFAI Supplier Invitrogen™ Supplier No. M3TNFAI

Description

Mouse Monoclonal Antibody

M3TNFAI targets TNF Alpha in ELISA, and WB applications and shows reactivity with Canine, Feline, Human, Non-human primate, and Porcine samples. The M3TNFAI immunogen is recombinant human TNF alpha from Pichia pastoris. M3TNFAI detects TNF Alpha which has a predicted molecular weight of approximately 26 kDa. Endotoxin levels are less than or equal to 10 μg/mL.

TNF alpha is a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophage and bind to its receptors, TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. TNF alpha is involved in the regulation of immune cells, cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. TNF alpha exists as a multimer of two, three, or five noncovalently linked units, but shows a single 17 kDa band following SDS-PAGE under non-reducing conditions. Knockout studies in mice also suggested the neuroprotective function of TNF alpha, and has been observed to causes tumor necrosis when injected into tumor-bearing mice. Other functions of TNF-alpha include its role in the immune response to bacterial, viral, parasitic and certain fungal infections, as well as its role in the necrosis of specific tumors. TNF alpha causes cytolysis or cytostasis of certain transformed cells, being synergistic with interferon-gamma in its cytotoxicity. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer.

Specifications

• Antigen: TNF alpha
• Applications: ELISA, Western Blot
• Classification: Monoclonal
• Clone: E10041
• Concentration: 0.5 mg/mL
• Conjugate: Unconjugated
• Formulation: PBS with 10% trehalose and no preservative
• Gene: Tnf
• Gene Accession No.: P01375, P19101, P23563, P51742
• Gene Alias: APC1 protein; Cachectin; C-domain 1; C-domain 2; cTNF; DADB-70P7.1; DIF; ICD1; ICD2; Intracellular domain 1; Intracellular domain 2; N-terminal fragment; NTF; RATTNF; Tnf; TNF alpha; TNF superfamily; TNF α; TNF, macrophage-derived; TNF, monocyte-derived; TNFA; TNF-a; TNFalpha; TNF-alpha; Tnfsf1a; TNFSF2; TNFα; TNLG1F; Tumor necrosis factor; tumor necrosis factor (TNF superfamily, member 2); tumor necrosis factor alpha; tumor necrosis factor alpha (cachetin); tumor necrosis factor alpha precursor; tumor necrosis factor ligand 1F; tumor necrosis factor ligand superfamily member 2; Tumor necrosis factor, membrane form; Tumor necrosis factor, soluble form; tumor necrosis factor-alpha; tumor necrosis factor-alpha precursor; tumor-necrosis factor; tumour necrosis factor
• Gene Symbols: Tnf
• Host Species: Mouse
• Immunogen: Recombinant human TNF alpha from Pichia pastoris.
• Purification Method: Protein G
• Quantity: 500 μg
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 397086, 403922, 493755, 7124
• Target Species: Canine, Feline, Human, Monkey, Pig
• Content And Storage: 4°C
• Product Type: Antibody
• Form: Lyophilized
• Isotype: IgG1

Safety and Handling

WARNING: Cancer - www.P65Warnings.ca.gov