Invitrogen™ TNF alpha Monoclonal Antibody (E10043)

Mouse Monoclonal Antibody

Supplier:  Invitrogen™ MTNFAI

Catalog No. ENMTNFAI

Description

MTNFAI targets TNF Alpha in ELISA, and Western blot applications and shows reactivity with Human samples. The MTNFAI antibody (clone E10043) has successfully been paired as the coating antibody in a sandwich ELISA with detection antibody M3TNFABI (biotinylated conjugate of clone E10041). The MTNFAI immunogen is recombinant human TNF alpha from Pichia pastoris. MTNFAI detects TNF Alpha which has a predicted molecular weight of approximately 26 kDa. Endotoxin levels are less than or equal to 10 μg/mL.

TNF alpha is a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophage and bind to its receptors, TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. TNF alpha is involved in the regulation of immune cells, cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. TNF alpha exists as a multimer of two, three, or five noncovalently linked units, but shows a single 17 kDa band following SDS-PAGE under non-reducing conditions. Knockout studies in mice also suggested the neuroprotective function of TNF alpha, and has been observed to causes tumor necrosis when injected into tumor-bearing mice. Other functions of TNF-alpha include its role in the immune response to bacterial, viral, parasitic and certain fungal infections, as well as its role in the necrosis of specific tumors. TNF alpha causes cytolysis or cytostasis of certain transformed cells, being synergistic with interferon-gamma in its cytotoxicity. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer.

Specifications

• Antigen: TNF alpha
• Classification: Monoclonal
• Concentration: 0.5 mg/mL
• Formulation: PBS with 10% trehalose and no preservative
• Gene Accession No.: P01375
• Gene Symbols: Tnf
• Immunogen: Recombinant human TNF alpha from Pichia pastoris.
• Quantity: 500 μg
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG1

• Applications: ELISA, Western Blot
• Clone: E10043
• Conjugate: Unconjugated
• Gene: Tnf
• Gene Alias: APC1 protein; Cachectin; C-domain 1; C-domain 2; cTNF; DADB-70P7.1; DIF; ICD1; ICD2; Intracellular domain 1; Intracellular domain 2; N-terminal fragment; NTF; RATTNF; Tnf; TNF alpha; TNF superfamily; TNF a; TNF, macrophage-derived; TNF, monocyte-derived; TNFA; TNF-a; TNFalpha; TNF-alpha; Tnfsf1a; TNFSF2; TNFa; TNLG1F; Tumor necrosis factor; tumor necrosis factor (TNF superfamily, member 2); tumor necrosis factor alpha; tumor necrosis factor alpha (cachetin); tumor necrosis factor alpha precursor; tumor necrosis factor ligand 1F; tumor necrosis factor ligand superfamily member 2; Tumor necrosis factor, membrane form; Tumor necrosis factor, soluble form; tumor necrosis factor-alpha; tumor necrosis factor-alpha precursor; tumor-necrosis factor; tumour necrosis factor
• Host Species: Mouse
• Purification Method: Protein G
• Regulatory Status: RUO
• Gene ID (Entrez): 7124
• Content And Storage: -20°C
• Form: Lyophilized

Safety and Handling

WARNING: Reproductive Harm - www.P65Warnings.ca.gov