Invitrogen™ TNF beta Monoclonal Antibody (359-81-11), APC, eBioscience™

Mouse Monoclonal Antibody

Supplier:  Invitrogen™ 17732781

Catalog No. 50-153-26

Description

Description: The 359-81-11 antibody reacts with Tumor Necrosis Factor-beta (TNF-beta), a potent lymphoid factor that exerts cytotoxic effects on a wide range of tumor cells and other target cells. Applications Reported:The 359-81-11 antibody has been reported for use in detection of human TNF beta by ELISA and intracellular staining for flow cytometric analysis. Has been tested by intracellular staining and flow cytometric analysis of TH1 polarized restimulated normal human peripheral blood cells. This can be used at less than or equal to 0.125 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser. Filtration: 0.2 μm post-manufacturing filtered.

TNF beta, a member of the tumor necrosis factor family, is a cytokine produced by lymphocytes. The protein is highly inducible, secreted, and forms heterotrimers with lymphotoxin-beta which anchor lymphotoxin-alpha to the cell surface. This protein also mediates a large variety of inflammatory, immunostimulatory, and antiviral responses, is involved in the formation of secondary lymphoid organs during development and plays a role in apoptosis. Genetic variations in this gene are associated with susceptibility to leprosy type 4 and psoriatic arthritis.

Specifications

• Antigen: TNF beta
• Classification: Monoclonal
• Concentration: 0.2 mg/mL
• Formulation: PBS with 0.09% sodium azide; pH 7.2
• Gene Accession No.: P01374
• Gene Symbols: LTA
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 4049
• Content And Storage: 4°C, store in dark, DO NOT FREEZE!
• Form: Liquid

• Applications: Flow Cytometry
• Clone: 359-81-11
• Conjugate: APC
• Gene: LTA
• Gene Alias: DAMA-25N12.13; hlb382; hypothetical protein; LT; LT[a]; LT-[a]; LTA; LTalpha; LT-alpha; Ltx; lymphotoxin A; lymphotoxin alpha; lymphotoxin alpha (TNF superfamily, member 1); lymphotoxin-alpha; lymphotoxin-alpha; lymphotoxin alpha; TNF b; TNF beta; TNF superfamily, member 1; TNF ß; Tnfb; TNFbeta; TNF-beta; TNF-N; Tnfsf1; Tnfsf1b; TNFß; TNLG1E; tumor necrosis factor beta; tumor necrosis factor ligand 1E; tumor necrosis factor ligand superfamily member 1
• Host Species: Mouse
• Quantity: 50 μg
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG1