TNF beta Polyclonal Antibody, PeproTech®, Invitrogen™

Rabbit Polyclonal Antibody

Supplier:  Pepro Tech Inc 500P31B100UG

Catalog No. 500P31B100

Description

AA Sequence of recombinant protein: MLPGVGLTPS AAQTARQHPK MHLAHSTLKP AAHLIGDPSK QNSLLWRANT DRAFLQDGFS LSNNSLLVPT SGIYFVYSQV VFSGKAYSPK ATSSPLYLAH EVQLFSSQYP FHVPLLSSQK MVYPGLQEPW LHSMYHGAAF QLTQGDQLST HTDGIPHLVL SPSTVFFGAF AL. Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human TNF-beta. Anti-Human TNF-beta-specific antibody was purified by affinity chromatography employing an immobilized Human TNF-beta matrix. Sandwich ELISA: To detect Human TNF-beta by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human TNF-beta (500-P31BBt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human TNF-beta. Western Blot: To detect Human TNF-beta by Western Blot analysis this antibody can be used at a concentration of 0.1- 0.2 μg/mL. When used in conjunction with compatible secondary reagents, the detection limit for Recombinant Human TNF-beta is 1.5-3.0 ng/lane, under either reducing non-reducing conditions.

TNF beta Polyclonal antibody specifically detects TNF beta in Human samples. It is validated for ELISA, Western Blot

Specifications

• Antigen: TNF beta
• Classification: Polyclonal
• Formulation: PBS with no preservative
• Gene Accession No.: P01374
• Host Species: Rabbit
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 4049
• Content And Storage: -20°C

• Applications: ELISA, Western Blot
• Conjugate: Unconjugated
• Gene: LTA
• Gene Alias: DAMA-25N12.13; hlb382; hypothetical protein; LT; LT[a]; LT-[a]; LTA; LTalpha; LT-alpha; Ltx; lymphotoxin A; lymphotoxin alpha; lymphotoxin alpha (TNF superfamily, member 1); lymphotoxin-alpha; lymphotoxin-alpha; lymphotoxin alpha; TNF b; TNF beta; TNF superfamily, member 1; TNF β; Tnfb; TNFbeta; TNF-beta; TNF-N; Tnfsf1; Tnfsf1b; TNFβ; TNLG1E; tumor necrosis factor beta; tumor necrosis factor ligand 1E; tumor necrosis factor ligand superfamily member 1
• Immunogen: E.coli-derived Recombinant Human TNF-beta
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Human
• Form: Lyophilized