Invitrogen™ TNFR2 (soluble) Polyclonal Antibody, Biotin, PeproTech®, Invitrogen™

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ 500P168BT25UG

Catalog No. 500P168BT25

Description

AA Sequence of recombinant protein: MAPEPGSTCR LREYYDQTAQ MCCSKCSPGQ HAKVFCTKTS DTVCDSCEDS TYTQLWNWVP ECLSCGSRCS SDQVETQACT REQNRICTCR PGWYCALSKQ EGCRLCAPLR KCRPGFGVAR PGTETSDVVC KPCAPGTFSN TTSSTDICRP HQICNVVAIP GNASMDAVCT STSP. Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human sTNF Receptor Type II. Anti-Human sTNF Receptor Type II-specific antibody was purified by affinity chromatography and then biotinylated. Sandwich ELISA: To detect hsTNF Receptor II by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1.0 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human sTNF Receptor II (500-P168) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant hsTNF Receptor II. Western Blot: To detect hsTNF Receptor II by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant hsTNF Receptor II is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions. 500-P168BT-1MG will be provided as 2 x 500 μg

TNFR2 (tumor necrosis factor receptor superfamily member 1B) is a receptor with high affinity for TNFSF2/TNF-alpha. It has a much lower affinity for homotrimeric TNFSF1/lymphotoxin-alpha. The TRAF1/TRAF2 complex recruits the apoptotic suppressors BIRC2 and BIRC3 to TNFRSF1B/TNFR2. TNFR2 mediates most of the metabolic effects of TNF-alpha.

Specifications

• Antigen: TNFR2 (soluble)
• Classification: Polyclonal
• Conjugate: Biotin
• Gene: Tnfrsf1b
• Gene Alias: CD120b; Etanercept; p75; p75 TNF receptor; p75 TNFR; p75TNFR; p80 TNF-alpha receptor; sCD120b; soluble CD120b; soluble TNFR1B variant 1; soluble TNFR2; sTNF R2; sTNFR 2; sTNFR2; TBP-2; TBPII; TNF receptor beta chain; TNF receptor superfamily member 1B; TNF-alphaR2; TNFalpha-R2; TNFBR; TNFR; TNFR II; Tnfr-1; TNFR1B; TNFR2; TNF-R2; Tnfr-2; TNF-R75; TNFR80; TNFRII; TNF-RII; TNF-R-II; TNFR-II; TNFRSF1B; tumor necrosis factor beta receptor; tumor necrosis factor binding protein 2; Tumor necrosis factor receptor; tumor necrosis factor receptor 2; Tumor necrosis factor receptor superfamily member 1B; Tumor necrosis factor receptor superfamily member 1b, membrane form; tumor necrosis factor receptor superfamily, member 1B; tumor necrosis factor receptor type II; Tumor necrosis factor-binding protein 2
• Host Species: Rabbit
• Purification Method: Antigen Affinity Chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 7133
• Content And Storage: -20°C
• Form: Lyophilized

• Applications: ELISA, Western Blot
• Concentration: 0.1-1.0 mg/mL
• Formulation: PBS with no preservative
• Gene Accession No.: P20333
• Gene Symbols: Tnfrsf1b
• Immunogen: E.coli-derived, 18.9 kDa Recombinant Human TNF Receptor Type II.
• Quantity: 25 μg
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody