Invitrogen™ Human TNFRSF6B (DcR3) ELISA Kit

Catalog No.	Quantity
50-274-1195	10 x 96 Tests
EHTNFRSF6B	96 Tests
50-255-0915	5 x 96 Tests

Catalog No. 50-274-1195 Supplier Invitrogen™ Supplier No. EHTNFRSF6BX10

Description

ELISA

The Human TNFRSF6B (DcR3) ELISA quantitates Hu DcR3 in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu DcR3. Principle of the method The Human DcR3 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Apoptosis, or programmed cell death, occurs during normal cellular differentiation and development of multicellular organisms. Apoptosis is induced by certain cytokines including TNF (tumor necrosis factor) and Fas ligand of the TNF family through their death domain containing receptors, TNFR1 (tumor necrosis factor receptor 1) and Fas. Cell death signals are transduced by death domain (DD)-containing adapter molecules and members of the ICE (interleukin-1-beta converting enzyme)/CED-3 (C. elegans death receptor-3) protease family. A novel DD-containing molecule was recently cloned from mouse, human and monkey and designated Daxx (death associated protein 6). Daxx binds specifically to the Fas death domain and enhances Fas induced apoptosis and activates the Jun N-terminal kinase (JNK) pathway. Daxx is widely expressed in fetal and adult human and mouse tissues indicating its important function in Fas signaling pathways.

Specifications

• Accession Number: O95407
• Assay Range: 0.328-80 ng/mL
• Assay Sensitivity: 0.3 ng/mL
• Calibration Range: 0.3 ng/mL to 80 ng/mL
• Assay: Solid-phase sandwich ELISA
• Conjugate: HRP
• Immunoassay Kit Format: Sandwich ELISA Kit
• Product Type: ELISA
• Protein Family: Other Proteins
• Sample Type: Plasma, Serum, Supernatant
• Specificity: This ELISA antibody pair detects human DcR3. Other species not determined.
• Sufficient For: 10 x 96 assays
• Target Species: Human
• For Use With (Application): ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
• For Use With (Equipment): Absorbance Microplate Reader
• Gene Alias: DcR3; Decoy receptor 3; decoy receptor 3 variant 1; decoy receptor 3 variant 2; decoy receptor for Fas ligand; DJ583P15.1.1; hCG_2044836; M68; M68E; OTTHUMP00000031583; TNF receptor superfamily member 6b; TNFRSF6B; TR6; tumor necrosis factor receptor superfamily member 6B; tumor necrosis factor receptor superfamily member 6b, decoy; tumor necrosis factor receptor superfamily, member 6b, decoy; UNQ186/PRO212DcR3; Decoy receptor 3; decoy receptor 3 variant 1; decoy receptor 3 variant 2; decoy receptor for Fas ligand; DJ583P15.1.1; hCG_2044836; M68; M68E; OTTHUMP00000031583; TNF receptor superfamily member 6b; TNFRSF6B; TR6; tumor necrosis factor receptor superfamily member 6B; tumor necrosis factor receptor superfamily member 6b, decoy; tumor necrosis factor receptor superfamily, member 6b, decoy; UNQ186/PRO212; DCR3; DJ583P15.1.1; M68; M68E; TR6
• Gene ID (Entrez): 8771
• Gene Symbol: TNFRSF6B
• Interassay CV: <12%
• Intraassay CV: <10%
• Kit Contents: Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Label or Dye: Biotin
• Protein Aliases: Decoy receptor 3, DcR3, Decoy receptor for Fas ligand, M68
• Quantity: 10 x 96 Tests
• Regulatory Status: RUO
• Research Discipline: Apoptosis
• Sample Volume: Plasma 50 μL, Serum 50 μL, Supernatant 100 μL
• Shipping Condition: Wet or Dry Ice
• Storage Requirements: Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C
• Synonym: hCG
• Target: DcR3
• Test Time: 1 hr 20 min
• Total Assay Time: 4 hr 45 min