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Invitrogen™ TOP10 Electrocomp™ Kit
Supplied at transformation efficiency of 1 × 109 cfu/μg supercoiled DNA for high-efficiency cloning and plasmid propagation
Supplier: Invitrogen™ C66455
Description
- Allows stable replication of high-copy number plasmids
- hsdR for efficient transformation of unmethylated DNA from PCR amplifications
- mcrA for efficient transformation of methylated DNA from genomic preparations
- lacZΔM15 for blue/white color screening of recombinant clones
- endA1 for cleaner preparations of DNA and better results in downstream applications due to elimination of non-specific digestion by Endonuclease I
- recA1 for reduced occurrence of non-specific recombination in cloned DNA
- Bacterial/Yeast Strain: TOP10
Applications:
Cloning, Transformation
Specifications
| Tube | |
| Electrocomp™ | |
| Contains enough electrocompetent cells for 12 x 40μL rxns or 25 x 20μL rxns | |
| Electrocompetent | |
| Yes | |
| Yes | |
| Yes | |
| May be used for plasmids > 20 kb | |
| Yes | |
| No | |
| > 1x10^10 |
| Transformation, Cloning | |
| Electroporator Kit | |
| 5 x 100 μL | |
| Not Suitable for cloning unstable DNA | |
| Lacks F' Episome | |
| TOP10 | |
| Not for ccdB vector propagation | |
| Not Suitable for preparing unmethylated DNA | |
| E. coli | |
| High Efficiency (> 10^9 cfu⁄µg) | |
| Not High-throughput Compatible (Manual) |
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Product Title
For Research Use Only. Not for use in diagnostic procedures.