TOX Monoclonal Antibody (TXRX10), PE, eBioscience™, Invitrogen™

Rat Monoclonal Antibody

Catalog Number	Mfr. No.	Quantity
50-245-515	Invitrogen™ 12650280	25 μg
50-245-516	Invitrogen™ 12650282	100 μg

Description

Description: This TXRX10 monoclonal antibody reacts with human and mouse TOX, which is also known as Thymocyte selection-associated HMG box protein. This DNA-binding nuclear factor is expressed in thymocytes, T lymphocytes, NK cells, and lymphoid tissue-inducer (LTi) cells. TOX expression in thymocytes is upregulated during beta-selection and positive selection. Studies using Tox knockout mice have demonstrated a critical role for TOX in CD4+ T cell development as a regulator of the CD4loCD8lo to CD4+CD8lo transition. Moreover, TOX has been shown to be involved in the development of NK and LTi cells. While its regulation remains unclear, TOX expression has been shown to be induced by calcineurin-mediated TCR signaling in thymocytes during positive selection. Applications Reported: This TXRX10 antibody has been reported for use in intracellular staining followed by flow cytometric analysis. Applications Tested: This TXRX10 antibody has been tested intracellular flow cytometric analysis of mouse thymocytes using the Foxp3 Buffers (cat. 00-5521) and protocol. This can be used at less than or equal to 0.5 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

TOX (thymocyte selection-associated high mobility group (HMG) box protein) is a member of the HMG box family of DNA-binding proteins and likely plays a role in the regulation of T-cell development. It is a 526 amino acid nuclear protein and the expression of TOX is upregulated by pre-T cell receptor (pre-TCR) and TCR activation in immature thymocytes. TOX-mediated positive selection is associated with up-regulation of RUNX3 and is calcineurin dependent. TOX-dependent transition to the CD4+CD8 stage is required for development of class II major histocompatibility complex-specific T cells.

Specifications

• Antigen: TOX
• Concentration: 0.2 mg/mL
• Classification: Monoclonal
• Form: Liquid
• Regulatory Status: RUO
• Formulation: PBS with 0.09% sodium azide; pH 7.2
• Gene Alias: 1700007F02Rik; KIAA0808; thymocyte selection associated high mobility group box; thymocyte selection-associated high mobility group box; thymocyte selection-associated high mobility group box protein TOX; thymocyte selection-associated HMG box; thymus high mobility group box protein TOX; TOX; TOX1
• Gene Symbols: TOX
• Primary or Secondary: Primary
• Content And Storage: 4°C, store in dark, DO NOT FREEZE!
• Gene: TOX

• Clone: TXRX10
• Applications: Flow Cytometry
• Conjugate: PE
• Host Species: Rat
• Target Species: Human, Mouse
• Gene Accession No.: O94900, Q66JW3
• Gene ID (Entrez): 252838, 9760
• Isotype: IgG2a κ
• Purification Method: Affinity chromatography
• Product Type: Antibody

For Research Use Only.