Invitrogen™ ToxBLAzer™ DualScreen Kit

Description

ToxBLAzer™ DualScreen Kit

ToxBLAzer™ DualScreen Kit provide the key advantage of combining the ratiometric reporter gene readout of beta-lactamase with a cytotoxicity readout in the same assay. Thus, ToxBLAzer™ DualScreen yields reporter readouts that normalize cytotoxic cellular responses, which is a necessity in screening applications which down regulate transcription. The ToxBLAzer™ substrate contains both the LiveBLAzer™-FRET B/G substrate and a proprietary cytotoxicity indicator. Both compounds readily enter the cell, where endogenous esterases rapidly convert them into fluorescent compounds Esterase activity and an intact cell membrane are required for detection of the indicator. The cytotoxicity indicator's fluorescence is red-shifted to ensure compatibility with the blue and green emissions of the LiveBLAzer™-FRET B/G substrate, allowing detection of both reporter gene activity and cytotoxicity in the same assay sample.

Advantages of ToxBLAzer™ DualScreen Kits:

Multiplexed readout results in rapid identification of false positives due to toxicity or lack of viable cells, thereby improving hit-picking efficiency

Ratiometric blue:green reporter readout reduces false hits by minimizing experimental noise

Single reagent addition eliminates need for follow up cytotoxicity assays, thereby increasing throughput while reducing sample and test compound consumption

Specifications

• For Use With (Equipment): Microplate Reader, Fluorometer, Flow Cytometer
• Product Line: ToxBLAzer™
• Content And Storage: ToxBLAzer™ DualScreen Loading Kits include ToxBLAzer™ substrate, DMSO (for Solution A), Solution B, and Solution C. ToxBLAzer™ substrate is provided lyophilized and should be stored at 20°C, dessicated and protected from light. DMSO (for Solution A), Solution B, and Solution C should be stored at 22-25°C, protected from direct light.
  
  The 200 μg kit is sufficient for 3,750 x 48 μl standard protocol well assays; the 5 mg kit is sufficient for 93,750 x 48 μl standard protocol well assays. Bulk quantities of loading kits are available upon request.
• Detection Method: Fluorescence
• Product Type: Dual Screen Kit
• Quantity: 200 μg
• Format: Kit
• Cell Permeability: Cell-Permeant
• Substrate: Beta-Lactamase Substrate

For Research Use Only. Not for use in diagnostic procedures.