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Thermo Scientific™ TR-FRET Coregulator Buffer H

Catalog No. PV4661
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Catalog No. PV4661 Supplier Thermo Scientific™ Supplier No. PV4661
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Description

A specialized nuclear receptor buffer that can be used with Invitrogen's collection of purified nuclear receptor proteins and assays.

A specialized nuclear receptor buffer that can be used with Invitrogen's collection of purified nuclear receptor proteins and assays.

TRUSTED_SUSTAINABILITY

Specifications

For Use With (Equipment) LanthaScreen™ Nuclear Receptor Cofactor Interaction Assays
Content And Storage TR-FRET Coregulator Buffer H may be shipped frozen or at room temperature. Store at room temperature (20°C to 30°C).
Shipping Condition Approved for shipment at Room Temperature or on Wet or Dry Ice
Quantity 25 mL

Frequently Asked Questions (FAQs)

I have no assay window in my TR-FRET assay. What is the reason?

The single most common reason that a TR-FRET assay fails is that an incorrect choice of emission filters was made. Unlike other fluorescent assays, the filters used in a TR-FRET assay must be exactly those that are recommended for your instrument as the emission filter choice can make or break the assay. The excitation filter has more of an impact on the assay window. Please refer to our instrument setup guides in our instrument compatibility portal (https://www.thermofisher.com/instrumentsetup). If your instrument is not listed there, please contact Drug Discovery Technical Support at drugdiscoverytech@thermofisher.com.

Note: Test your microplate reader's TR-FRET setup before you begin any work with your assay. Using reagents that you have already purchased for your assay, you can test the microplate reader's TR-FRET setup. Please refer to the Terbium (Tb) Assay (http://tools.thermofisher.com/content/sfs/manuals/LanthaScreen_Tb_Instrument_Control_Application_Note17Sep10.pdf) and Europium (Eu) Assay (http://tools.thermofisher.com/content/sfs/manuals/Eu_Instrument_Control_AppNote_12Nov10.pdf) Application Notes for plate reader setup.

For Research Use Only. Not for use in diagnostic procedures.

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