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Invitrogen™ TRA-1-81 (Podocalyxin) Monoclonal Antibody (TRA-1-81), APC, eBioscience™
Mouse Monoclonal Antibody
Supplier: Invitrogen™ 17888342

Description
Description: The TRA-1-81 antibody recognizes a protein expressed on undifferentiated human embryonic stem cells (ES), embyronal carcinoma cells (EC), and embryonic germ cells (EG). Like other stem cell specific markers, the epitope recognized by the TRA-1-81 antibody is lost upon cell differentiation. The TRA-1-81 epitope is resistant to neuraminidase digestion, unlike the epitope recognized by the related TRA-1-60 antibody. The TRA-1-81 antibody is known to specifically recognize a carbohydrate epitope on a keratan sulfated glycoprotein recently identified as podocalyxin, a member of the CD34-related family of sialomucins. Podocalyxin is a transmembrane glycoprotein originally identified on epithelial glomerular cells known as podocytes, and the protein has also been implicated in the development of aggressiveness in a variety of cancers, including breast and prostate cancer. Applications Reported: This TRA-1-81 antibody has been reported for use in flow cytometric analysis. Applications Tested: This TRA-1-81 antibody has been pre-titrated and tested by flow cytometric analysis of 2102Ep human embryonal carcinoma cells. This can be used at 5 μL (0.25 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser. Filtration: 0.2 μm post-manufacturing fi...
TRA-1-81 is a cell surface antigen expressed along with SSEA-3, SSEA-4 and TRA-1-60 in human embryonic stem cells, embryonal carcinoma cells and induced pluripotent stem cells (iPS). These surface markers are down-regulated during the differentiation process. In contrast, SSEA-1 is absent in undifferentiated human stem cells but is present on the cell surface after retinoic acid mediated differentiation.
Specifications
TRA-1-81 (Podocalyxin) | |
Monoclonal | |
5 μL/Test | |
PBS with BSA and 0.09% sodium azide; pH 7.2 | |
Mouse | |
100 Tests | |
Primary | |
4°C, store in dark, DO NOT FREEZE! | |
Liquid |
Flow Cytometry | |
TRA-1-81 | |
APC | |
GCTM-2 antigen; Gp200; PC; PCLP; PCLP1; PCLP-1; Podocalyxin; podocalyxin like; podocalyxin-like; podocalyxin-like protein 1; PODXL; TRA180; tra1-81 | |
Affinity chromatography | |
RUO | |
Human | |
Antibody | |
IgM |
Safety and Handling
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