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Invitrogen™ Human Transferrin ELISA Kit

Catalog No. EHTFX10
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10 x 96 Tests
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EHTFX10 10 x 96 Tests
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ELISA

Transferrins (Tf) are an approximately 80kDa blood plasma glycoprotein synthesized by the liver. They are iron binding transport proteins which can bind two Fe(3+) ions in association with the binding of an anion, usually bicarbonate. It is responsible for the transport of iron from sites of absorption and heme degradation to those of storage and utilization. Transferrin is the primary blood iron transport protein and under normal conditions; approximately one-third of total blood transferrin contains bound iron. Measurement of blood transferrin levels can be used as an indicator for blood iron-carrying capacity and abnormalities of iron metabolism such as anaemia, iron overload and haemochromatosis.Serum transferrin may also have a further role in stimulating cell proliferation.
TRUSTED_SUSTAINABILITY

Specifications

Accession Number P02787
Assay Range 1.029 to 750 ng/mL
Assay Sensitivity 1 ng/mL
Conjugate HRP
Product Type ELISA
Sample Type Plasma, Serum, Supernatant
For Use With (Equipment) Colorimetric Microplate Reader
Gene ID (Entrez) 7018
Gene Symbol Tf
Interassay CV <12%
Intraassay CV <10
Kit Contents Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
Label or Dye HRP
Quantity 10 x 96 Tests
Regulatory Status RUO
Sample Volume Plasma 2 μL, Serum 2 μL, Supernatant 100 μL
Storage Requirements 2°C to 8°C
Target Transferrin
Test Time 1 hrs 20 mins
Total Assay Time 4 hrs 45 mins
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