Novus Biologicals™ TrkB Antibody Pair [HRP]

Description

Mouse Monoclonal Antibody Pair

This solid phase sandwich ELISA utilizes a monoclonal antibody specific for Human TrkB.

Specifications

• Assay Range: 10.98 pg/mL
• Assay Sensitivity: 10.98-700 pg/mL
• Assay: Sandwich ELISA
• Conjugate: HRP
• Product Type: Antibody Pairs
• Specificity: This solid phase sandwich ELISA utilizes a monoclonal antibody specific for Human TrkB.
• Target Species: Human
• For Use With (Application): Sandwich ELISA
• Gene Alias: BDNF/NT-3 growth factors receptor, EC 2.7.10, EC 2.7.10.1, GP145-TrkB, Neurotrophic tyrosine kinase receptor type 2, neurotrophic tyrosine kinase, receptor, type 2, trk-B, TrkB tyrosine kinase, TRKBBDNF-tropomyosine receptor kinase B, tyrosine kinase receptor B
• Gene ID (Entrez): 4915
• Gene Symbol: NTRK2
• Host Species: Mouse
• Kit Contents:
  Standard: Each vial contains 28 ng of recombinant Human TrkB.
  Capture Antibody: 1 mg/mL of mouse anti-Human TrkB monoclonal antibody (in PBS, pH 7.4).
  Detection Antibody: 0.2 mg/mL of mouse anti-Human TrkB monoclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % HRP-Protector, pH 7.4, store at 4C).
• Quantity: 15 Plates
• Regulatory Status: RUO
• Research Discipline: GPCR, Neuroendocrinology, Oncogenes, Phospho Specific, Protein Kinase, Signal Transduction
• Storage Requirements: Storage is content dependent.
• Target: TrkB
• Technique: 1. Capture Antibody: Dilute to a working concentration of 2 μg/mL in PBS before coating. 2. Detection Antibody: Dilute to working concentration of 0.3 μg/mL in detection antibody dilution buffer before use. 3. Standard: Reconstitute with 1 ml detection antibody dilution buffer. After reconstitution, store at -20 to -80°C in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 700 pg/mL is recommended.