Invitrogen™ Trop2 (EGP-1) Monoclonal Antibody (MR54), Alexa Fluor™ 488, eBioscience™

Mouse Monoclonal Antibody

Supplier:  Invitrogen™ 53602482

Catalog No. 50-246-084

Description

Description: This MR54 monoclonal antibody reacts with human Trop-2, which is also known as Tumor-Associated Calcium Signal Transducer 2 (TACSTD2) and EGP-1. This type I transmembrane glycoprotein is expressed on trophoblast (syncytiotrophoblast and cytotrophoblast), epithelial, and most human carcinoma cells. Trop-2 plays a role in calcium-mediated signaling. Trop-2 has also been reported as an oncogene, with expression being correlated with tumorigenesis, metastasis, and anchorage-independent growth. Applications Reported: This MR54 antibody has been reported for use in flow cytometric analysis, immunohistochemical staining of formalin-fixed paraffin embedded (FFPE) tissue sections (IHC-P), and immunocytochemistry (ICC). Applications Tested: This MR54 antibody has been tested by flow cytometry and immunocytochemistry on MCF7 cells. This can be used at less than of equal to 10 μg/mL. For immunohistochemistry of formalin-fixed paraffin embedded tissue, either low or high pH antigen retrieval can be used, although high pH antigen retrieval results in better staining intensity. Is is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Excitation: 488 nm; Emission: 519 nm; Laser: Blue Laser. Filtration: 0.2 μm post-manufacturing filtered.

TROP2 is a carcinoma-associated antigen defined by the monoclonal antibody GA733. This antigen is a member of a family including at least two type I membrane proteins. It transduces an intracellular calcium signal and acts as a cell surface receptor. Mutations of its gene result in gelatinous drop-like corneal dystrophy, an autosomal recessive disorder characterized by severe corneal amyloidosis leading to blindness.

Specifications

• Antigen: Trop2 (EGP-1)
• Classification: Monoclonal
• Concentration: 0.5 mg/mL
• Formulation: PBS with 0.09% sodium azide; pH 7.2
• Gene Accession No.: P09758
• Gene Symbols: TACSTD2
• Purification Method: Affinity Chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 4070
• Content And Storage: 4°C, store in dark, DO NOT FREEZE!
• Form: Liquid

• Applications: Flow Cytometry, Immunohistochemistry (Paraffin), Immunocytochemistry
• Clone: MR54
• Conjugate: Alexa Fluor 488
• Gene: TACSTD2
• Gene Alias: 40kD glycoprotein, identified by monoclonal antibody GA733; C80403; cell surface glycoprotein TROP2; cell surface glycoprotein Trop-2; EGP1; EGP-1; epithelial glycoprotein-1; GA733; GA7331; GA733-1; gastrointestinal tumor-associated antigen GA7331; GP50; Ly97; lymphocyte antigen 97; M1S1; Membrane component chromosome 1 surface marker 1; membrane component, chromosome 1, surface marker 1; pancreatic carcinoma marker protein GA7331; pancreatic carcinoma marker protein GA733-1; parturition-related protein 1; Prp1; TACD2; TACSTD2; Trop2; truncated TACSTD2; tumor-associated calcium signal transducer 2; tunor-associated calcium signal transducer 2
• Host Species: Mouse
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG2a κ

Safety and Handling

WARNING: Cancer - www.P65Warnings.ca.gov