Invitrogen™ TrueCut™ HiFi Cas9 Protein (5 μg/μL)

Description

A high fidelity CRISPR Cas9 protein variant engineered to demonstrate superior off-target profiles, while preserving maximum on-target editing efficiency. TrueCut HiFi Cas9 Protein is ideal for applications that are sensitive to off-target events and when more precise editing is required.

Invitrogen TrueCut HiFi Cas9 Protein is a high fidelity CRISPR Cas9 protein variant engineered to demonstrate superior off-target profiles, while preserving maximum on-target editing efficiency.

Features include:

• Near complete elimination of off-target events in all tested cell lines including standard, immune, primary, and stem
• Maintains maximum on-target editing efficiency compared to TrueCut Cas9 Protein v2
• Transfection-ready using either electroporation or lipid-mediated transfection reagents
• Manufactured under strict ISO-13485 quality standards
• Ideal for applications that are sensitive to off-target events and when more precise editing is required
• Eliminates time-consuming cloning steps

Available in two Concentrations:

• 1 μg/μL: for use in standard editing scenarios
• 5 μg/μL: for optimized editing conditions in more difficult scenarios such as in primary or embryonic cells, microinjection, or when screening multiple gRNA sequences at a time
• For custom sizes or concentrations, please inquire

Options for obtaining CRISPR gRNA for use with TrueCut Cas9 Protein v2:

• Order pre-designed TrueGuide Synthetic gRNAs for high-efficiency knock out in human or mouse
• Submit your own design for custom synthesis of CRISPR sgRNA
• Use our free TrueDesign CRISPR Genome Editor software for easy design and ordering of custom gRNA for precise genome editing

Specifications

• Concentration: 5 μg/μL
• Purity: ≥90%
• Sensitive to Heat Inactivation: Yes
• Source: E. coli
• Quantity: 100 μg
• Product Type: HiFi Cas9
• Form: Liquid