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Invitrogen™ TrueTag™ Donor DNA Kit, BFP

The Invitrogen TrueTag Donor DNA Kit, BFP, provides a rapid solution to drive high-efficiency gene tagging (up to 100% edited cells) with transfection-ready donor DNA for genome editing experiments.

Supplier:  Invitrogen™ A53806

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Catalog No. A53806


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Description

Description

  • A rapid solution to drive high-efficiency gene tagging (up to 100% edited cells) with transfection-ready donor DNA for genome editing experiments
  • Kit provides everything needed to produce a high-quality DNA donor to tag the gene with a selection marker and BFP
  • Simply provide two locus-specific primers to add homology arms, PCR-amplify, and purify for a transfection-ready donor in hours, not days
  • One-step PCR amplification to generate donor templates – no cloning required
  • Enrich up to 100% BFP-tagged cells

Each kit is designed to enable users to:

  • Tag the carboxy- (C-) or amino- (N-) terminus of the gene of interest
  • Choose from puromycin or blasticidin selection markers – whatever best suits the cell model
  • Easily remove antibiotic resistance markers with Cre/Lox following cell enrichment

Included in the kit:

  • Four linear donor templates: N-terminus/puromycin resistance, N-terminus/blasticidin resistance, C-terminus/puromycin resistance, C-terminus/blasticidin resistance
  • Phusion Flash High-Fidelity PCR Master Mix
  • PCR cleanup columns and buffers
  • Positive control primers to tag human beta-actin (ACTB)

Intuitive software to rapidly design gene-specific reagents

Target-specific gene editing reagents are required for proper knock-in of your fluorescent tag. The Invitrogen TrueDesign Genome Editor web-based software does the design work for you:

  1. Identifies target gene transcript
  2. Designates insertion site
  3. Designs and enables easy ordering of all necessary components

Required reagents available via TrueDesign software (not included in kit):

  • CRISPR guide RNA or TALENs designed adjacent to N- or C-terminus
  • Oligos to PCR-amplify the donor and add appropriate homology arms
  • Sequencing primers for assessment of CRISPR-Cas9 cleavage efficiency
  • Verification primers to validate proper insertion of the TrueTag DNA Donor

Also required and available for purchase separately or as part of TrueDesign workflow:

  • TrueCut Cas9 Protein v2, TrueCut HiFi Cas9 Protein, or GeneART CRISPR mRNA
  • CRISPRMAX Transfection Reagent or MessengerMAX Transfection Reagent
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Specifications

Specifications

Cellular Imaging
10 reactions
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For Research Use Only. Not for use in diagnostic procedures.