MP Biomedicals™ Trypsin, Porcine Pancreas

Description

Typical use for this product is in removing adherent cells from a culture surface. The concentration of trypsin necessary to dislodge cells from their substrate is dependent primarily on the cell type and the age of the culture.

• CAS Number: 9002-07-7
• Molecular Weight: Approximately 23,000 or 22000 (for human)
• Composition: Trypsin is composed of two subunits, a-trypsin and b-trypsin. a-Trypsin is composed of two peptide chains and b-trypsin is composed of one chain.
• Activity: One BAEE unit will produce a DA253 of 0.001 per minute at pH 7.6 at 25°C using BAEE as substrate. Reaction volume = 3.2mL (1 cm light path).
  One TAME unit hydrolyzes 1 umole of p-toluene-sulfonyl-L-arginine methyl ester (TAME) per minute at 25°C, pH 8.2, in the presence of 0.001 M calcium ion.
  One USP trypsin unit is the activity causing a change in absorbance of 0.003 per minute under the conditions specified.
• Activity Conversion:1 TAME unit = 19.2 USP or NF units = 57.5 BAEE Units
• Composition: Trypsin is composed of two subunits, a-trypsin and b-trypsin. a-Trypsin is composed of two peptide chains and b-trypsin is composed of one chain.

Specifications

• For Use With (Application): Release adherent cells from tissue culture plates for passaging
• Sample Type: Porcine
• Content And Storage: 4°C
• Form: Powder
• Quantity: 25 g
• Product Type: Trypsin