Invitrogen™ TTC30A Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA521835

Catalog No. PIPA521835

Description

Recommended positive controls: 293T, A431, HepG2, Molt-4. Predicted reactivity: Rat (90%), Xenopus laevis (88%), Chicken (85%), Bovine (93%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

The TTC30A gene encodes for the Tetratricopeptide Repeat Domain 30A, which is part of the intraflagellar transport (IFT) complex B, critical for ciliary function. TTC30A and its paralog TTC30B are involved in the stability and functioning of the IFT complex. These proteins play a redundant role in maintaining cilia integrity, necessary for proper cellular signaling and homeostasis. Disruption of TTC30A, through CRISPR/Cas9 gene editing, has shown to impair ciliogenesis, leading to developmental defects such as limb malformations and polycystic kidney disease due to altered tubulin acetylation and ciliary transport. This highlights the importance of TTC30A in cellular and developmental processes.

Specifications

• Antigen: TTC30A
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: TTC30A
• Gene Alias: IFT70A; tetratricopeptide repeat domain 30A; tetratricopeptide repeat domain 30B; tetratricopeptide repeat protein 30A; TPR repeat protein 30A; TTC30A
• Host Species: Rabbit
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 92104
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid

• Applications: Immunohistochemistry (Paraffin), Western Blot
• Concentration: 1 mg/mL
• Formulation: 0.1M tris glycine with 10% glycerol and 0.01% thimerosal; pH 7
• Gene Accession No.: Q86WT1
• Gene Symbols: TTC30A
• Immunogen: Recombinant protein encompassing a sequence within the center region of human TTC30A. The exact sequence is proprietary.
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG