Invitrogen™ UBC13 Monoclonal Antibody (4E 11)

Mouse Monoclonal Antibody

Supplier:  Invitrogen™ 371100

Catalog No. 37-110-0

Description

UBC13 Monoclonal Antibody for Western Blot, ICC/IF, ELISA

The UBE2V1-UBE2N and UBE2V2-UBE2N heterodimers catalyze the synthesis of non-canonical 'Lys-63'-linked polyubiquitin chains. This type of polyubiquitination does not lead to protein degradation by the proteasome. Mediates transcriptional activation of target genes. Plays a role in the control of progress through the cell cycle and differentiation. Plays a role in the error-free DNA repair pathway and contributes to the survival of cells after DNA damage. Acts together with the E3 ligases, HLTF and SHPRH, in the 'Lys-63'-linked poly-ubiquitination of PCNA upon genotoxic stress, which is required for DNA repair. Appears to act together with E3 ligase RNF5 in the 'Lys-63'-linked polyubiquitination of JKAMP thereby regulating JKAMP function by decreasing its association with components of the proteasome and ERAD. Promotes TRIM5 capsid-specific restriction activity and the UBE2V1-UBE2N heterodimer acts in concert with TRIM5 to generate 'Lys-63'-linked polyubiquitin chains which activate the MAP3K7/TAK1 complex which in turn results in the induction and expression of NF-kappa-B and MAPK-responsive inflammatory genes. Together with RNF135 and UB2V1, catalyzes the viral RNA-dependent 'Lys-63'-linked polyubiquitination of RIG-I/DDX58 to activate the downstream signaling pathway that leads to interferon beta production. UBE2V1-UBE2N together with TRAF3IP2 E3 ubiquitin ligase mediate 'Lys-63'-linked polyubiquitination of TRAF6, a component of IL17A-mediated signaling pathway. (Uniprot).

Specifications

• Antigen: UBC13
• Classification: Monoclonal
• Concentration: 0.5 mg/mL
• Formulation: PBS with 0.1% sodium azide; pH 7.4
• Gene Accession No.: P61088, P61089, Q9EQX9
• Gene Symbols: UBE2N
• Immunogen: Recombinant human Ubc13.
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat
• Product Type: Antibody
• Isotype: IgG1 κ

• Applications: ELISA, Western Blot, Immunocytochemistry
• Clone: 4E 11
• Conjugate: Unconjugated
• Gene: UBE2N
• Gene Alias: 1500026J17Rik; AL022654; BB101821; bendless protein; bendless-like ubiquitin conjugating enzyme; bendless-like ubiquitin-conjugating enzyme; Blu; E2 ubiquitin-conjugating enzyme N; epididymis secretory protein Li 71; HEL-S-71; MGC131857; MGC8489; UBC13; UbcH13; UbcH-ben; UBCHBEN; UBC13; UBE2N; Ubiquitin carrier protein N; ubiquitin conjugating enzyme E2 N; ubiquitin conjugating enzyme E2N; ubiquitin-conjugating enzyme E2 N; ubiquitin-conjugating enzyme E2N; ubiquitin-conjugating enzyme E2N (homologous to yeast UBC13); ubiquitin-conjugating enzyme E2N (UBC13 homolog, yeast); ubiquitin-protein ligase N; yeast UBC13 homolog
• Host Species: Mouse
• Purification Method: Protein A
• Regulatory Status: RUO
• Gene ID (Entrez): 116725, 7334, 93765
• Content And Storage: -20°C
• Form: Liquid