Invitrogen™ UCP1 Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA124894

Catalog No. PA124894

Description

PA1-24894 detects UCP1 from human, mouse, and rat samples, and does not cross-react with UCP2 or UCP3. PA1-24894 is expected to cross react with bovine (93% conserved), canine (93% conserved), Macaque monkey (100% conserved), rabbit (93% conserved), and ovine (93% conserved) due to sequence homology. By Western Blot this antibody detects a band of approximately 32 kDa. Positive Control: Brown adipose tissue.

Mitochondrial uncoupling proteins are members of the family of mitochondrial anion carrier proteins. UCPs separate oxidative phosphorylation from ATP synthesis with energy dissipated as heat, also referred to as the mitochondrial proton leak. UCPs facilitate the transfer of anions from the inner to the outer mitochondrial membrane and the return transfer of protons from the outer to the inner mitochondrial membrane. They also reduce the mitochondrial membrane potential in mammalian cells. Tissue specificity occurs for the different UCPs and the exact methods of how UCPs transfer H+/OH- are not known. UCPs contain the three homologous protein domains of MACPs. This gene is expressed only in brown adipose tissue, a specialized tissue which functions to produce heat.

Specifications

• Antigen: UCP1
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: Ucp1
• Gene Alias: AI385626; HGNC:12517; mitochondrial brown fat uncoupling protein 1; SLC25A7; solute carrier family 25 member 7; Thermogenin; Ucp; UCP 1; Ucp1; Ucpa; uncoupling protein; uncoupling protein 1; uncoupling protein 1 (mitochondrial, proton carrier); uncoupling protein 1, mitochondrial; uncoupling protein, mitochondrial; Uncp; unnamed protein product
• Host Species: Rabbit
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 101089511, 22227, 24860, 7350
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid

• Applications: Immunohistochemistry (Paraffin), Western Blot
• Concentration: 1.3 mg/mL
• Formulation: PBS with 1% BSA and 15mM sodium azide; pH 7.4
• Gene Accession No.: P04633, P12242, P25874
• Gene Symbols: Ucp1
• Immunogen: Synthetic peptide corresponding to human UCP1 sequence (amino acids 145-159) with N-terminal cysteine added, conjugated to KLH.
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat, Feline, Ferret
• Product Type: Antibody
• Isotype: IgG