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R&D Systems™ Universal Chemiluminescent PARP Assay - Histone Coated Wells

Chemiluminescent assay kit for candidate inhibitor screening and determination of IC50 values of PARP inhibitors.

Manufacturer:  R&D Systems™ 4676096K

Catalog No. 46-760-96K


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Description

Description

HT Universal 96-well PARP Assay Kits measure the incorporation of biotinylated poly(ADP-ribose) onto histone proteins in a strip well format. This assay is ideal for the screening of PARP inhibitors and determining IC50 values. Important features of the assay include: 1) chemiluminescent, non-radioactive format; 2) higher throughput 96 test size; and 3) sensitivity down to 0.01 Units of PARP per well.

Kit Contents:

  • PARP-HSA, 10 U/µL 50 µL
  • 20X PARP Buffer (2 vials) 2 x 500 µL
  • 10X PARP Cocktail (300 µlL)
  • Histone-Coated White Strip Wells (96 Wells)
  • 200 mM 3-Aminobenzamide (60 µL)
  • 10X Strep-Diluent (2 mL)
  • Strep-HRP (30 µL)
  • PeroxyGlow™ A (6 mL)
  • PeroxyGlow™ B (6 mL)
  • 10X Activated DNA (300 µL)
Specifications

Specifications

96 Tests
Store the unopened product at -70°C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.
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