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MilliporeSigma™ Universal EZ-TFA Chemiluminescent Transcription Factor Assay

Catalog No. 70600 Shop All MilliporeSigma Products
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96 Assays
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Catalog No. Quantity
70-600 96 Assays
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Catalog No. 70-600 Supplier MilliporeSigma™ Supplier No. 70600

Description

Includes

  • Streptavidin Coated Chemiluminescent Plate
  • Blocking Reagent
  • 10X Annealing Buffer
  • Chemiluminescent Detection Reagent
  • Chemiluminescent Reaction Buffer
  • TFA Buffer, 5X
  • Gt X Rb, HRP Secondary Antibody
  • Gt X Ms, HRP Secondary Antibody

Fast, sensitive method to detect specific transcription factor DNA binding activity in whole cell or nuclear extracts

  • Provided in a 96-well format
  • When the Capture Probe, a double stranded biotinylated oligonucleotide is incubated together, the active form of NFkB contained in the nuclear extract binds to its consensus sequence
  • After incubation, the extract/probe/buffer mixture is then directly transferred to the streptavidin coated plate
  • The biotinylated double stranded oligonucleotide bound by active NFkB protein is immobilized and any inactive, unbound material is washed away
  • The bound NFkB transcription factor subunits, p50 and p65, are detected with specific primary antibodies, a Rabbit anti-NFkB p50 (active form) and a Rabbit anti-NFkB p65
  • An HRP-conjugated secondary antibody is then used for detection and provides sensitive colorimetric detection that can be read in a spectrophotometric plate reader
TRUSTED_SUSTAINABILITY

Specifications

Content And Storage Store at 4°C:
  • Transcription Factor Assay Chemiluminescent Plate
  • Blocking Reagent
  • 10X Annealing Buffer
  • Chemiluminescent Detection Reagent
  • Chemiluminescent Rea
Detection Method Chemiluminescence
For Use With (Application) ELISA, Activity Assay
Quantity 96 Assays
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