Sigma Aldrich Fine Chemicals Biosciences Uracil DNA Glycosylase hea

Supplier:  Sigma Aldrich Fine Chemicals Biosciences UDGHLRO

Uracil-DNA Glycosylase heat-labile contains the equally named enzyme found in the marine bacterium BMTU 3346. Like the UNG from E. coli it hydrolyzes uracil-glycosidic bonds in single- or double-stranded DNA excising uracil and creating alkali-sensitive abasic sites in the DNA. These abasic sites can be hydrolyzed by endonuclease heat or alkali treatment. Depending on how the DNA is prepared Uracil-DNA Glycosylase can be used to achieve general site-specific or strand-specific U-DNA cleavage. This enzyme shows lower thermostability and is therefore easier to inactivate.Enzyme CharacteristicsThe BMTU 3346 enzyme is inactivated more quickly (2 minutes at 95C) than the corresponding enzyme from E. coli (10 minutes at 95C). It has also been reported that UNG from E. coli remains partially active leading to the degradation of the dU-containing PCR product. In contrast the heat-labile UNG does not degrade dU-PCR products within at least several hours of incubation at 2 to 8C. Theref

Catalog No. 50-290-8100