Invitrogen™ Human Uromodulin (UMOD) ELISA Kit

Catalog No.	Quantity
EHUMODX10	10 x 96 Tests

Catalog No. EHUMODX10 Supplier Invitrogen™ Supplier No. EHUMODX10

Description

ELISA

This gene encodes uromodulin, the most abundant protein in normal urine. Its excretion in urine follows proteolytic cleavage of the ectodomain of its glycosyl phosphatidylinosital-anchored counterpart that is situated on the luminal cell surface of the loop of Henle. Uromodulin may act as a constitutive inhibitor of calcium crystallization in renal fluids. Excretion of uromodulin in urine may provide defense against urinary tract infections caused by uropathogenic bacteria. Defects in this gene are associated with the autosomal dominant renal disorders medullary cystic kidney disease-2 (MCKD2) and familial juvenile hyperuricemic nephropathy (FJHN). These disorders are characterized by juvenile onset of hyperuricemia, gout, and progressive renal failure. While several transcript variants may exist for this gene, the full-length natures of only two have been described to date. These two represent the major variants of this gene and encode the same isoform.

Specifications

• Accession Number: P07911
• Assay Range: 40.96 to 10,000 pg/mL
• Assay Sensitivity: 40 pg/mL
• Conjugate: HRP
• Product Type: ELISA
• Sample Type: Plasma, Serum, Supernatant
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene ID (Entrez): 7369
• Gene Symbol: Umod
• Interassay CV: <12%
• Intraassay CV: <10
• Kit Contents: Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Label or Dye: HRP
• Quantity: 10 x 96 Tests
• Regulatory Status: RUO
• Sample Volume: Plasma 1 μL, Serum 1 μL, Supernatant 100 μL
• Storage Requirements: 2°C to 8°C
• Target: Uromodulin
• Test Time: 1 hrs 20 mins
• Total Assay Time: 4 hrs 45 mins