Novus Biologicals™ VEGF 164 Antibody Pair [HRP]

Polyclonal/Monoclonal Antibody Pair

Supplier:  Novus Biologicals™ NBP27961915PLATES

Catalog No. NB010277

Description

This solid phase sandwich ELISA utilizes an antibody specific for Rat VEGF 164.

Specifications

• Assay Range: 23.44 pg/mL
• Assay: Sandwich ELISA
• Immunoassay Kit Format: Sandwich ELISA Kit
• Specificity: This solid phase sandwich ELISA utilizes an antibody specific for Rat VEGF 164.
• For Use With (Application): Sandwich ELISA
• Gene Symbol: VEGFA
• Kit Contents:
• Detection Antibody: 0.2 mg/mL of rabbit anti-VEGF 164 polyclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % HRP-Protector, pH 7.4, store at 4C).
• Capture Antibody: 1 mg/mL of rabbit anti-Rat VEGF 164 monoclonal antibody (in PBS, pH 7.4).
• Standard: Each vial contains 32 ng of recombinant Rat VEGF 164.
• Regulatory Status: RUO
• Storage Requirements: Storage is content dependent.
• Technique: 1. Capture Antibody: Dilute to a working concentration of 2 μg/mL in PBS before coating. 2. Detection Antibody: Dilute to working concentration of 0.5 μg/mL in detection antibody dilution buffer before use. 3. Standard: Reconstitute with 1 ml detection antibody dilution buffer. After reconstitution, store at -20 to -80°C in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 1500 pg/mL is recommended.

• Assay Sensitivity: 23.44-1500 pg/mL
• Conjugate: HRP
• Product Type: Antibody Pairs
• Target Species: Rat
• Gene ID (Entrez): 7422
• Host Species: Rabbit
• Quantity: 15 Plates
• Research Discipline: Biologically Active Proteins
• Target: VEGF 164