Invitrogen™ VEGF-165 Polyclonal Antibody, PeproTech®, Invitrogen™

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ 500P27550UG

Catalog No. 500P27550UG

Description

AA Sequence of recombinant protein: MAPTTEGEQK AHEVVKFMDV YQRSYCRPIE TLVDIFQEYP DEIEYIFKPS CVPLMRCAGC CNDEALECVP TSESNVTMQI MRIKPHQSQH IGEMSFLQHS RCECRPKKDR TKPEKHCEPC SERRKHLFVQ DPQTCKCSCK NTDSRCKARQ LELNERTCRC DKPRR. Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Rat VEGF 165. Anti-Rat VEGF 165-specific antibody was purified by affinity chromatography employing an immobilized Rat VEGF 165 matrix. Sandwich ELISA: To detect Rat VEGF 165 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Rat VEGF 165 (500-P275Bt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Rat VEGF 165. Western Blot: To detect Rat VEGF 165 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Rat VEGF 165 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

The vascular endothelial growth factor (VEGF) family currently includes VEGF-A, VEGF-B, VEGF-C, VEGF-D, VEGF-E, and PIGF. VEGF and its receptor system have been shown to be the fundamental regulators in the cell signaling of angiogenesis. Most tumors have the absolute requirement of angiogenesis and VEGF has been described as the most potent angiogenic cytokine linked to this process. To date 5 different isoforms of VEGF have been described. These isoforms are generated as the result of alternative splicing from a single VEGF gene. These various isoforms have been shown to bind to two tyrosine-kinase receptors flt-1 (VEGFR-1) and flk-1/KDR (VEGFR-2), which have been found to be expressed almost exclusively on endothelial cells.

Specifications

• Antigen: VEGF-165
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: Vegfa
• Gene Alias: H-VEGF-165; MVCD1; M-VEGF-165; vascular endothelial growth factor 165b; vascular endothelial growth factor A; vascular endothelial growth factor A121; vascular endothelial growth factor A165; vascular permeability factor; VEGF; VEGF164; VEGF165; Vegfa; VEGF-A; VPF
• Host Species: Rabbit
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 83785
• Content And Storage: -20°C
• Form: Lyophilized

• Applications: ELISA, Western Blot
• Concentration: 0.1-1.0 mg/mL
• Formulation: PBS with no preservative
• Gene Accession No.: P16612
• Gene Symbols: Vegfa
• Immunogen: E.coli-derived Recombinant Rat VEGF 165.
• Quantity: 50 μg
• Primary or Secondary: Primary
• Target Species: Rat
• Product Type: Antibody