Thermo Scientific™ Verso 1-Step RT-PCR Kit ReddyMix, with ThermoPrime Taq

Thermo Scientific™ Verso 1-Step RT-PCR Master Mixes are optimized for the detection and analysis of RNA templates in a single step.

Supplier:  Thermo Scientific™ AB1454LDB

Catalog No. AB1454LDB

Description

Thermo Scientific™ Verso™ 1-Step RT-PCR Master Mixes are optimized for the detection and analysis of RNA templates in a single step.

The Verso 1-Step RT-PCR system is composed of a processive RT enzyme that can effectively transcribe through difficult RNA secondary structure, and a buffer formulation that ensures high performance from both the reverse transcription step and the PCR step. This robust system delivers one-step convenience while maintaining high sensitivity and accuracy. Available in standard and hot-start formats.

• Reverse transcription and amplification steps are carried out in a single reaction tube delivering convenient, fast analysis
• Highly sensitive with a broad dynamic range: can accurately detect RNA input from 1pg to 1μg
• High-affinity RT enzyme effectively transcribes through difficult RNA sequences
• Optimized 1-Step buffer maximizes performance from both RT and PCR steps
• Wide working temperature range (42° to 57°C) for success with GC-rich and other difficult templates
• Included RT Enhancer prevents genomic DNA carryover, eliminating the need for separate DNase I treatment
• RNase Inhibitor included in RT enzyme mix

Format Options:

1-Step RT-PCR Master Mix w/ThermoPrime Taq

• Contains ThermoPrime Taq DNA polymerase for highly sensitive detection and analysis of RNA

1-Step RT-PCR ReddyMix Master Mix w/ThermoPrime Taq

• Contains ThermoPrime Taq DNA polymerase for highly sensitive detection and analysis of RNA
• ReddyMix Master Mix contains an inert red tracking dye and gel-loading precipitant
• Reaction mix can be directly loaded onto agarose gels post-PCR, without the addition of loading dye
• Red dye allows for easy sample visualization during reaction setup

1-Step RT-PCR Master Mix w/Thermo-Start Taq (Hot Start)

• Contains Thermo-Start Taq DNA polymerase for hot start PCR activation to deliver maximum sensitivity and specificity
• Safe for room temperature reaction setup
• Thermo-Start Taq remains completely inactive until it is switched on by a high temperature incubation step at the start of the PCR reaction
• Hot-start technology prevents non-specific amplification during reaction setup and the RT step, eliminating smearing and non-target bands

Verso RNA Control Kit

• Intended for use as a positive control to monitor the performance of all RT-PCR components
• Kit includes a 3.6kb RNA synthesized according to the sequence derived from bacteriophage MS2 and primers designed to generate a 522 bp product

Compatible with:

Verso cDNA Synthesis Kits

Recommended for:

1-Step RT-PCR

Specifications

• Content And Storage:

  • Verso Enzyme Mix
  • 2X Master Mix
  • RT Enhancer
  
  Store at –20°C.
  
  

• Format: Kit
• Polymerase: ThermoPrime Taq DNA Polymerase
• Optimal Reaction Temperature: 42°C to 57°C
• Ribonuclease H Activity: Yes
• For Use With (Application): RT-PCR
• No. of Reactions: 200 Reactions
• Reagent Type: Reverse Transcription
• Starting Material: RNA

• Detection Method: Gel Electrophoresis
• PCR Method: 1-step RT-PCR
• Technique: Reverse Transcription
• Reverse Transcriptase: Verso
• Shipping Condition: Dry Ice
• Final Product Type: PCR Amplified cDNA
• Reaction Format: Premixed Components
• Size (Final Product): Up to 11 kb

Purchase of this product includes an immunity from suit under patents specified in the product insert to use only the amount purchased for the purchaser's own internal research. No other patent rights are conveyed expressly, by implication, or by estoppel. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.