Invitrogen™ VHL Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA527322

Catalog No. PIPA527322

Description

Recommended positive controls: Myc-DDK-tagged VHL-transfected 293T. Predicted reactivity: Mouse (93%), Rat (91%), Dog (95%), Bovine (96%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Von Hippel-Lindau syndrome (VHL) is a dominantly inherited familial cancer syndrome predisposing to a variety of malignant and benign tumors. A germline mutation of this gene is the basis of familial inheritance of VHL syndrome. The protein encoded by this gene is a component of the protein complex that includes elongin B, elongin C, and cullin-2, and possesses ubiquitin ligase E3 activity. This protein is involved in the ubiquitination and degradation of hypoxia-inducible-factor (HIF), which is a transcription factor that plays a central role in the regulation of gene expression by oxygen. RNA polymerase II subunit POLR2G/RPB7 is also reported to be a target of this protein. Alternatively spliced transcript variants encoding distinct isoforms have been observed.

Specifications

• Antigen: VHL
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: VHL
• Gene Alias: elongin binding protein; HRCA1; Protein G7; pVHL; RCA1; VHL; VHL1; VHL19; Vhlh; VHLp18(MEA); Von Hippel Lindau; von hippel-lindau; von Hippel-Lindau disease tumor suppressor; von Hippel-Lindau syndrome homolog; von Hippel-Lindau syndrome protein homolog; von Hippel-Lindau tumor suppressor; von Hippel-Lindau tumor suppressor, E3 ubiquitin protein ligase
• Host Species: Rabbit
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 22346, 7428
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid

• Applications: Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot, Immunocytochemistry
• Concentration: 0.6 mg/mL
• Formulation: PBS with 20% glycerol and 0.025% ProClin 300; pH 7
• Gene Accession No.: P40337, P40338
• Gene Symbols: VHL
• Immunogen: Recombinant protein encompassing a sequence within the center region of human Von Hippel Lindau. The exact sequence is proprietary.
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human, Mouse
• Product Type: Antibody
• Isotype: IgG

Safety and Handling

WARNING: Cancer - www.P65Warnings.ca.gov