Vimentin Monoclonal Antibody (V9), Biotin, Invitrogen™

Mouse Monoclonal Antibody

Supplier:  Thermo Scientific MA511880

Catalog No. PIMA511880

Description

MA5-11880 targets Vimentin in immunohistochemistry (paraffin) applications and shows reactivity with Bovine, Canine, Chicken, Equine, Feline, Gerbil, Hamster, Human, mouse, Non-human primate, Porcine, Rabbit, and Rat samples. The MA5-11880 immunogen is purified vimentin from pig eye lens. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH6.0, for 10-20 minutes followed by cooling at RT for 20 min.

Vimentin is a developmentally regulated intermediate filament protein (IFP) found in cells of mesenchymal origin. It is believed to be involved with the intracellular transport of proteins between the nucleus and plasma membrane. Unlike other IFP proteins, vimentin is expressed, along with desmin, during the early stages of cellular development. During the development process, vimentin is exchanged for new, tissue-specific IFPs. Vimentin has been implicated to be involved in the rate of steroid synthesis via its role as a storage network for steroidogenic cholesterol containing lipid droplets. Vimentin phosphorylation by a protein kinase causes the breakdown of intermediate filaments and activation of an ATP and myosin light chain dependent contractile event. This results in cytoskeletal changes that facilitate the interaction of the lipid droplets within mitochondria, and subsequent transport of cholesterol to the organelles leading to an increase in steroid synthesis.

Specifications

• Antigen: Vimentin
• Classification: Monoclonal
• Concentration: 0.2 mg/mL
• Formulation: PBS with 0.2% BSA and 0.09% sodium azide; pH 7.4
• Gene Accession No.: P02543, P08670, P09654, P20152, P31000, P48616, P48670
• Gene Symbols: Vim
• Immunogen: Purified vimentin from pig eye lens
• Quantity: 500 μL
• Primary or Secondary: Primary
• Target Species: Bovine, Canine, Chicken, Equine, Feline, Gerbil, Hamster, Human, Mouse, Non-human Primate, Pig, Rabbit, Rat
• Product Type: Antibody
• Isotype: IgG1 κ

• Applications: Immunohistochemistry (Paraffin)
• Clone: V9
• Conjugate: Biotin
• Gene: Vim
• Gene Alias: cb28; class-III intermediate microfilament; CTRCT30; epididymis luminal protein 113; FLJ36605; HEL113; intermediate filament; intermediate filament protein; RP11-124N14.1; similar to vimentin; VIM; VIME; Vimentin; vimentin C-terminus
• Host Species: Mouse
• Purification Method: Protein G
• Regulatory Status: RUO
• Gene ID (Entrez): 100008924, 100056088, 100522394, 100689099, 101091951, 22352, 280955, 420519, 477991, 7431, 81818
• Content And Storage: 4° C
• Form: Liquid