Invitrogen™ Vimentin Monoclonal Antibody (VI-01)

Mouse Monoclonal Antibody

Supplier:  Invitrogen™ MA119168

Catalog No. 01-675-938

Description

This antibody reacts with vimentin, a 57 kDa intermediate filament intracellular protein expressed in variety of mesenchymal and mesodermal cell types. Cross-reactivity was found with smooth muscle desmin. Immunocytochemistry: Staining technique: (a) fix cells for 10 min in methanol at -20°C and for 6 min in acetone at -20°C; (b) fix cells directly in methanol for 10 min at -20°C or in acetone for 10 min at -20°C. Positive control: 3T3 murine Swiss albino fibroblast cell line, RBL rat basophilic leukemia cell line. Flow cytometry: Intracellular staining.

Vimentin is a developmentally regulated intermediate filament protein (IFP) found in cells of mesenchymal origin. It is believed to be involved with the intracellular transport of proteins between the nucleus and plasma membrane. Unlike other IFP proteins, vimentin is expressed, along with desmin, during the early stages of cellular development. During the development process, vimentin is exchanged for new, tissue-specific IFPs. Vimentin has been implicated to be involved in the rate of steroid synthesis via its role as a storage network for steroidogenic cholesterol containing lipid droplets. Vimentin phosphorylation by a protein kinase causes the breakdown of intermediate filaments and activation of an ATP and myosin light chain dependent contractile event. This results in cytoskeletal changes that facilitate the interaction of the lipid droplets within mitochondria, and subsequent transport of cholesterol to the organelles leading to an increase in steroid synthesis.

Specifications

• Antigen: Vimentin
• Classification: Monoclonal
• Concentration: 1 mg/mL
• Formulation: TBS with 15mM sodium azide; pH 8
• Gene Accession No.: P02543, P08670, P20152
• Gene Symbols: Vim
• Immunogen: Pellet of pig brain cold stable proteins after depolymerization of Microtubules.
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Pig, Mammalia, Rat
• Product Type: Antibody
• Isotype: IgM

• Applications: Flow Cytometry, Immunohistochemistry (Paraffin), Western Blot, Immunocytochemistry
• Clone: VI-01
• Conjugate: Unconjugated
• Gene: Vim
• Gene Alias: cb28; class-III intermediate microfilament; CTRCT30; epididymis luminal protein 113; FLJ36605; HEL113; intermediate filament; intermediate filament protein; RP11-124N14.1; similar to vimentin; VIM; VIME; Vimentin; vimentin C-terminus
• Host Species: Mouse
• Purification Method: Sequential Chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 100522394, 22352, 7431
• Content And Storage: 4°C, do not freeze
• Form: Liquid