Invitrogen™ Vimentin Monoclonal Antibody (VI-RE/1), PE

Mouse Monoclonal Antibody

Supplier:  Invitrogen™ MA119656

Catalog No. 01-676-683

Description

This antibody reacts with human vimentin, a 57 kDa intermediate filament intracellular protein expressed on a wide variety of mesenchymal and mesodermal cell types. This antibody will not cross-react with porcine or mouse.

Vimentin is a developmentally regulated intermediate filament protein (IFP) found in cells of mesenchymal origin. It is believed to be involved with the intracellular transport of proteins between the nucleus and plasma membrane. Unlike other IFP proteins, vimentin is expressed, along with desmin, during the early stages of cellular development. During the development process, vimentin is exchanged for new, tissue-specific IFPs. Vimentin has been implicated to be involved in the rate of steroid synthesis via its role as a storage network for steroidogenic cholesterol containing lipid droplets. Vimentin phosphorylation by a protein kinase causes the breakdown of intermediate filaments and activation of an ATP and myosin light chain dependent contractile event. This results in cytoskeletal changes that facilitate the interaction of the lipid droplets within mitochondria, and subsequent transport of cholesterol to the organelles leading to an increase in steroid synthesis.

Specifications

• Antigen: Vimentin
• Classification: Monoclonal
• Concentration: 0.1 mg/mL
• Formulation: PBS with 0.2% BSA and 15mM sodium azide; pH 7.4
• Gene Accession No.: P08670
• Gene Symbols: Vim
• Immunogen: Bacterially expressed full length human vimentin.
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG1

• Applications: Flow Cytometry
• Clone: VI-RE/1
• Conjugate: PE
• Gene: Vim
• Gene Alias: cb28; class-III intermediate microfilament; CTRCT30; epididymis luminal protein 113; FLJ36605; HEL113; intermediate filament; intermediate filament protein; RP11-124N14.1; similar to vimentin; VIM; VIME; Vimentin; vimentin C-terminus
• Host Species: Mouse
• Purification Method: Size-exclusion Chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 7431
• Content And Storage: 4°C, store in dark, DO NOT FREEZE!
• Form: Liquid

Safety and Handling

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