Invitrogen™ Vimentin Polyclonal Antibody

Chicken Polyclonal Antibody

Supplier:  Invitrogen™ PA116759

Catalog No. APPA6759

Description

Vimentin Polyclonal Antibody for Western Blot, ICC/IF, IHC (F), IHC (P)

Vimentin is a developmentally regulated intermediate filament protein (IFP) found in cells of mesenchymal origin. It is believed to be involved with the intracellular transport of proteins between the nucleus and plasma membrane. Unlike other IFP proteins, vimentin is expressed, along with desmin, during the early stages of cellular development. During the development process, vimentin is exchanged for new, tissue-specific IFPs. Vimentin has been implicated to be involved in the rate of steroid synthesis via its role as a storage network for steroidogenic cholesterol containing lipid droplets. Vimentin phosphorylation by a protein kinase causes the breakdown of intermediate filaments and activation of an ATP and myosin light chain dependent contractile event. This results in cytoskeletal changes that facilitate the interaction of the lipid droplets within mitochondria, and subsequent transport of cholesterol to the organelles leading to an increase in steroid synthesis.

Specifications

• Antigen: Vimentin
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: Vim
• Gene Alias: cb28; class-III intermediate microfilament; CTRCT30; epididymis luminal protein 113; FLJ36605; HEL113; intermediate filament; intermediate filament protein; RP11-124N14.1; similar to vimentin; VIM; VIME; Vimentin; vimentin C-terminus
• Host Species: Chicken
• Purification Method: IgY fraction
• Regulatory Status: RUO
• Gene ID (Entrez): 100056088, 100522394, 22352, 280955, 420519, 477991, 7431, 81818
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid

• Applications: Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin), Western Blot, Immunocytochemistry
• Concentration: 16.6 mg/mL
• Formulation: antibody with 0.035% sodium azide
• Gene Accession No.: P02543, P08670, P09654, P20152, P31000, P48616
• Gene Symbols: Vim
• Immunogen: The immunogen used to generate our antibody was recombinant vimentin purified from E. coli.
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat, Canine, Bovine, Pig, Horse, Chicken
• Product Type: Antibody
• Isotype: IgY