Invitrogen™ Vimentin Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA527231

Catalog No. PIPA527231

Description

Recommended positive controls: 293T, HeLa, MDA-MB-231, U87-MG, SK-N-SH, IMR32, SK-N-AS, NIH-3T3, A549. Predicted reactivity: Mouse (98%), Rat (98%), Xenopus laevis (85%), Dog (98%), Chicken (92%), Chimpanzee (99%), Bovine (98%), Guinea pig (97%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Vimentin is a developmentally regulated intermediate filament protein (IFP) found in cells of mesenchymal origin. It is believed to be involved with the intracellular transport of proteins between the nucleus and plasma membrane. Unlike other IFP proteins, vimentin is expressed, along with desmin, during the early stages of cellular development. During the development process, vimentin is exchanged for new, tissue-specific IFPs. Vimentin has been implicated to be involved in the rate of steroid synthesis via its role as a storage network for steroidogenic cholesterol containing lipid droplets. Vimentin phosphorylation by a protein kinase causes the breakdown of intermediate filaments and activation of an ATP and myosin light chain dependent contractile event. This results in cytoskeletal changes that facilitate the interaction of the lipid droplets within mitochondria, and subsequent transport of cholesterol to the organelles leading to an increase in steroid synthesis.

Specifications

• Antigen: Vimentin
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: Vim
• Gene Alias: cb28; class-III intermediate microfilament; CTRCT30; epididymis luminal protein 113; FLJ36605; HEL113; intermediate filament; intermediate filament protein; RP11-124N14.1; similar to vimentin; VIM; VIME; Vimentin; vimentin C-terminus
• Host Species: Rabbit
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 22352, 7431, 81818
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid

• Applications: ELISA, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot, Immunocytochemistry
• Concentration: 0.16 mg/mL
• Formulation: PBS with 1% BSA, 20% glycerol and 0.025% ProClin 300; pH 7
• Gene Accession No.: P08670, P20152, P31000
• Gene Symbols: Vim
• Immunogen: Recombinant protein fragment corresponding to a region within amino acids 61 and 426 of Human Vimentin.
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat
• Product Type: Antibody
• Isotype: IgG

Safety and Handling

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