Invitrogen™ Vimentin Polyclonal Antibody

Goat Polyclonal Antibody

Supplier:  Invitrogen™ PA5142829

Catalog No. PIPA5142829

Description

This antibody is tested in Peptide ELISA: antibody detection limit dilution 1:4,000.

Vimentin is a developmentally regulated intermediate filament protein (IFP) found in cells of mesenchymal origin. It is believed to be involved with the intracellular transport of proteins between the nucleus and plasma membrane. Unlike other IFP proteins, vimentin is expressed, along with desmin, during the early stages of cellular development. During the development process, vimentin is exchanged for new, tissue-specific IFPs. Vimentin has been implicated to be involved in the rate of steroid synthesis via its role as a storage network for steroidogenic cholesterol containing lipid droplets. Vimentin phosphorylation by a protein kinase causes the breakdown of intermediate filaments and activation of an ATP and myosin light chain dependent contractile event. This results in cytoskeletal changes that facilitate the interaction of the lipid droplets within mitochondria, and subsequent transport of cholesterol to the organelles leading to an increase in steroid synthesis.

Specifications

• Antigen: Vimentin
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: Vim
• Gene Alias: cb28; class-III intermediate microfilament; CTRCT30; epididymis luminal protein 113; FLJ36605; HEL113; intermediate filament; intermediate filament protein; RP11-124N14.1; similar to vimentin; VIM; VIME; Vimentin; vimentin C-terminus
• Host Species: Goat
• Purification Method: Ammonium sulfate precipitation
• Regulatory Status: RUO
• Gene ID (Entrez): 22352, 7431, 81818
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Form: Liquid

• Applications: Flow Cytometry, Immunohistochemistry (Paraffin), Western Blot, Immunocytochemistry
• Concentration: 0.5 mg/mL
• Formulation: TBS with 0.5% BSA and 0.02% sodium azide; pH 7.3
• Gene Accession No.: P08670, P20152, P31000
• Gene Symbols: Vim
• Immunogen: Peptide with sequence C-QVINETSQHHDDLE.
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat
• Product Type: Antibody
• Isotype: IgG

Safety and Handling

WARNING: Cancer - www.P65Warnings.ca.gov