VISTA Monoclonal Antibody (MH5A), Functional Grade, eBioscience™, Invitrogen™

Armenian Hamster Monoclonal Antibody

Catalog Number	Mfr. No.	Quantity
16-591-985	Invitrogen™ 16591985	500 μg

Description

Description: The monoclonal antibody MH5A reacts with a homolog of PD-1 (PD-1H) also known as VISTA. PD-1H is a 309 aa type I transmembrane protein that belongs to the CD28 superfamily. This cell surface receptor is expressed on nearly all hematopoietic cells except B cells. VISTA negatively regulates T cell responses by suppressing early TCR activation, inhibiting cell proliferation, and suppressing TH1 cytokine production. The MH5A antibody has been reported to inhibit T cell infiltration in graft-versus-host disease mouse models. Applications Reported: This MH5A antibody has been reported for use in flow cytometric analysis. Applications Tested: This MH5A antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.25 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Storage and handling: Use in a sterile environment. Filtration: 0.2 μm post-manufacturing filtered. Purity: Greater than 90%, as determined by SDS-PAGE. Endotoxin Level: Less than 0.001 ng/μg antibody, as determined by LAL assay. Aggregation: Less than 10%, as determined by HPLC.

VISTA (V-domain Ig containing suppressor of T-cell activation), also known as B7-H5 or PD-1H, is a product of the VSIR gene. Although VISTA bears sequence homology to PD-L1 it has a different expression pattern. In both mouse and humans, VISTA is expressed predominantly on hematopoietic cells with the highest densities on myeloid cells, and lower levels on T cells. It is usually absent on B cells. VISTA expressed by antigen presenting cells has been shown to directly suppress proliferation and cytokine production of CD4 and CD8 T cells, making it a target for cancer immunotherapy. The reported ligands of VISTA include CD28H, VSIG-3 and VSIG-8.

Specifications

• Antigen: VISTA
• Concentration: 1 mg/mL
• Conjugate: Functional Grade
• Host Species: Armenian Hamster
• Target Species: Mouse
• Gene Accession No.: Q9D659
• Isotype: IgG
• Purification Method: Affinity Chromatography
• Product Type: Antibody

• Clone: MH5A
• Classification: Monoclonal
• Form: Liquid
• Regulatory Status: RUO
• Formulation: PBS with no preservative; pH 7.2
• Gene ID (Entrez): 74048
• Primary or Secondary: Primary
• Content And Storage: 4°C
• Gene: VSIR