Invitrogen™ Vitronectin Monoclonal Antibody (HV2)

Mouse Monoclonal Antibody

Supplier:  Invitrogen™ CSI0030202

Catalog No. CSI0030202

Description

CSI 003-02-02 is highly specific for vitronectin. There is no evidence for cross-reactivity with other connective tissue proteins (fibronectin, elastin, collagen, laminin). CSI 003-02-02 is human-specific (no reactivity with cat, dog, cow, sheep, goat, pig, rabbit, horse). CSI 003-02-02 has been successfully used in ELISA, immunohistochemistry, and Western blotting techniques. CSI 003-02-02 can be used to purify vitronectin from human plasma by affinity chromatography. It can also be used to quantitatively affinity-deplete human plasma or serum of vitronectin. It partially denatures vitronectin upon antibody binding. It binds equally well to native and denatured vitronectin and can be used to quantitate vitronectin in human plasma in a sandwich ELISA with CSI 003-02-02. It does not interfere with the binding of any known vitronectin ligands. The epitope of CSI 003-02-02 is located in the connecting region. Binding can be competed selectively by a peptide comprising aa 121-133. NOTE: Concentration is lot-dependent and can vary from 0.85-1.15 mg/mL.

VTN is a member of the pexin family. This protein is found in serum and tissues and promotes cell adhesion and spreading, inhibits the membrane-damaging effect of the terminal cytolytic complement pathway, and binds to several serpin serine protease inhibitors. The protein is a secreted protein and exists in either a single chain form or a clipped, two chain form held together by a disulfide bond.

Specifications

• Antigen: Vitronectin
• Classification: Monoclonal
• Concentration: 1.0 mg/mL
• Formulation: PBS with 15mM sodium azide; pH 7.4
• Gene Accession No.: P04004
• Gene Symbols: VTN
• Immunogen: Human vitronectin purified from plasma by heparin-affinity chromatography.
• Quantity: 400 μL
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG2b κ

• Applications: ELISA, Immunohistochemistry (Paraffin), Western Blot, Immunocytochemistry
• Clone: HV2
• Conjugate: Unconjugated
• Gene: VTN
• Gene Alias: Aa1018; AI256434; complement S-protein; epibolin; extracellular matrix protein; glycoprotein 66; serum spreading factor; serum-spreading factor; somatomedin B; Somatomedin-B; S-protein; V75; Vitronectin; vitronectin (serum spreading factor, somatomedin B, complement S-protein); Vitronectin V10 subunit; Vitronectin V65 subunit; VN; VNT; VTN
• Host Species: Mouse
• Purification Method: Protein A
• Regulatory Status: RUO
• Gene ID (Entrez): 7448
• Content And Storage: 4°C, store in dark
• Form: Liquid

Safety and Handling

WARNING: Reproductive Harm - www.P65Warnings.ca.gov