Sartorius Vivaflow™ 200 Crossflow Cassettes

Catalog No.	Material (Membrane)	MWCO
14-558-322	PES	100 kDa
14-558-320	PES	30 kDa
14-558-318	PES	10 kDa
14-558-315	Cellulose	10 kDa
14-558-321	PES	50 kDa
14-558-317	Cellulose	30 kDa
14-558-319	PES	5 kDa
14-558-323	PES	0.2 μm
14-558-314	Cellulose	100 kDa
14-558-316	Cellulose	5 kDa

Catalog No. 14-558-322 Supplier Sartorius Supplier No. VF20P4

Description

Offers a standard for ease of use, reliability and flexibility unrivalled by other filtration systems. Sartorius Vivaflow 200 Crossflow Cassettes are ready to use, reusable laboratory crossflow cassettes for concentrating up to 5L samples. Available in different MWCOs and membrane types for different application needs.

Rapid, Efficient Concentration

• Optimally suited for cell culture supernatant concentration, virus concentration and water concentration
• Utilizes a 200cm² PES or Hydrosart membrane for high flow rate
• Thin-channel, flip-flow recirculation path provides high crossflow velocities with minimum pump speed
• Fast concentration using one or two cassettes
  • A single module typically concentrates 1L by a factor of 50 in less than 30 minutes
  • Two modules used in parallel concentrates 5L by a factor of 50 in under 75 minutes
• Near total recovery of the concentrate is achieved with a single rinse
• A 500ml sealed diafiltration reservoir can be added for rapid and convenient buffer exchange

Convenient and Easy to Use

• One unit comes with all necessary accessories (except pump and pump head)
  • Includes tubing, pressure indicator, flow restrictor and high pressure pump tubing
  • Required equipment: peristaltic pump with a size 16 pump head
• Can be easily cleaned and re-used several times
• Clear housing for process monitoring
• Ready-to-use, disposable cassettes
• Easily set up two cassettes to run simultaneously for faster concentration
  • Use a pre-assembled Y connector with the correct tubing sizes and fittings
  • Size 16 tubing is used for connecting the two Vivaflow 200 units
  • Size 15 tubing to connect to the pump, with a size 15 pump head

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Specifications

• Filter Type: Ultrafiltration
• Format: Cassette
• For Use With (Equipment): Labscale TFF System
• Material: Acrylic
• Material (Membrane): PES
• MWCO: 100 kDa
• Sample Volume: Up to 2.5 L
• Sterility: Non-sterile
• Filtration Area: 200 cm²
• For Use With (Application): Cell culture supernatant concentration, virus concentration and water concentration
• Includes: Pressure indicator, flow restrictor and size 16 PVC peristaltic tubing and fittings
• Max. Pressure: 60psi
• Pump Flow Rate: 200 to 400mL/min.
• Quantity: 1 Each
• Temperature (English) Max.: 140°F
• Temperature (Metric) Max: 60°C
• Type: Crossflow Cassette
• Volume (Metric): 5 L
• Volume (Metric) Hold Up: 5.3 mL
• Dimensions (L x W x H): 4.96 x 1.49 x 5.43 in. (12.6 x 3.8 x 13.8cm)
• Length (Metric): 12.6 cm
• Width (Metric): 3.8 cm
• Width (Metric) Channel: 1 cm
• Height (Metric): 13.8 cm
• Height (Metric) Channel: 0.04 cm