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Molecular Probes™ Vybrant™ DyeCycle™ Violet/SYTOX™ AADvanced™ Apoptosis Kit, for flow cytometry

Catalog No. 501121506
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Catalog No. 50-112-1506 Supplier Molecular Probes™ Supplier No. A35135
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Description

Vybrant™ DyeCycle™ Violet/SYTOX™ AADvanced™ Apoptosis Kit, for flow cytometry

The Vybrant™ DyeCycle™ Violet/SYTOX™ AADvanced™ apoptosis kit is used for the identification of apoptotic cells. This kit provides a rapid and convenient assay based upon fluorescence analysis of the compacted state of the nuclear chromatin in apoptotic cells. The kit contains solutions of the cell-permeant Vybrant™ DyeCycle™ Violet stain (Ex/Em max ∼370/440nm) which is excited with UV or violet laser and SYTOX™ AADvanced™ (Ex/Em max ∼546/650 nm) which is excited with a 488 nm laser. SYTOX™ AADvanced™ is used to detect changes in permeability of the plasma membrane therefore allowing identification of dead cells. The staining pattern resulting from simultaneous use of both dyes in combination make it possible to distinguish normal, apoptotic, and dead cell populations by flow cytometry.

View a selection guide for all apoptosis assays for flow cytometry.
TRUSTED_SUSTAINABILITY

Specifications

Quantity 1 kit
Format Tube
Product Type Apoptosis Kit
Content And Storage Contains 1 vial of Vybrant™ DyeCycle™ Violet (50 μL, 5mM solution in water); 1 vial SYTOX™ AADvanced™ stain (100 μg); and 1 vial of DMSO (200 μL).

Storage 2-6°C
Flow Cytometer Laser Lines 405, 488
Excitation/Emission SYTOX™ AADvanced™: 546⁄647, Vybrant™ DyeCycle™ Violet: 369⁄437
No. of Reactions 200
Shipping Condition Room Temperature
Product Line Vybrant
Detection Method Fluorescence
For Use With (Application) Flow Cytometry
For Use With (Equipment) Flow Cytometer
Label or Dye Vybrant DyeCycle Violet, SYTOX AADvanced Dead Cell Stain
Label Type Other Label(s) or Dye(s)
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Safety and Handling

WARNING: Reproductive Harm - www.P65Warnings.ca.gov

For Research Use Only. Not for use in diagnostic procedures.

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