Molecular Probes™ Vybrant™ DyeCycle™ Violet/SYTOX™ AADvanced™ Apoptosis Kit, for flow cytometry

Description

Vybrant™ DyeCycle™ Violet/SYTOX™ AADvanced™ Apoptosis Kit, for flow cytometry

The Vybrant™ DyeCycle™ Violet/SYTOX™ AADvanced™ apoptosis kit is used for the identification of apoptotic cells. This kit provides a rapid and convenient assay based upon fluorescence analysis of the compacted state of the nuclear chromatin in apoptotic cells. The kit contains solutions of the cell-permeant Vybrant™ DyeCycle™ Violet stain (Ex/Em max ∼370/440nm) which is excited with UV or violet laser and SYTOX™ AADvanced™ (Ex/Em max ∼546/650 nm) which is excited with a 488 nm laser. SYTOX™ AADvanced™ is used to detect changes in permeability of the plasma membrane therefore allowing identification of dead cells. The staining pattern resulting from simultaneous use of both dyes in combination make it possible to distinguish normal, apoptotic, and dead cell populations by flow cytometry.

View a selection guide for all apoptosis assays for flow cytometry.

Specifications

• Quantity: 1 kit
• Format: Tube
• Product Type: Apoptosis Kit
• Content And Storage: Contains 1 vial of Vybrant™ DyeCycle™ Violet (50 μL, 5mM solution in water); 1 vial SYTOX™ AADvanced™ stain (100 μg); and 1 vial of DMSO (200 μL).
  
  Storage 2-6°C
• Flow Cytometer Laser Lines: 405, 488
• Excitation/Emission: SYTOX™ AADvanced™: 546⁄647, Vybrant™ DyeCycle™ Violet: 369⁄437
• No. of Reactions: 200
• Shipping Condition: Room Temperature
• Product Line: Vybrant™
• Detection Method: Fluorescence
• For Use With (Application): Flow Cytometry
• For Use With (Equipment): Flow Cytometer
• Label or Dye: Vybrant DyeCycle Violet, SYTOX AADvanced Dead Cell Stain
• Label Type: Other Label(s) or Dye(s)

Safety and Handling

WARNING: Reproductive Harm - www.P65Warnings.ca.gov

For Research Use Only. Not for use in diagnostic procedures.