Invitrogen™ Novex™ Tris-Glycine Mini Protein Gel Kits, 4–20%, 1.0 mm, WedgeWell™ format

Catalog No.	Wells	Quantity
50-008-19211	10-well	30 Gels (3 Boxes)
XP04200BOX	10-well	10 Gels (1 Box)
XP04200PK2	10-well	2 Gels (1 Box)
XP04202BOX	12-well	10 Gels (1 Box)
50-008-19212	12-well	30 Gels (3 Boxes)
XP04205BOX	15-well	10 Gels (1 Box)
50-008-19213	15-well	30 Gels (3 Boxes)

Catalog No. 50-008-19211 Supplier Invitrogen™ Supplier No. XP04200BOXPR

Description

Novex Tris-Glycine Mini Gels, WedgeWell format, are polyacrylamide gels that provide reproducible separation of a wide range of proteins into well-resolved bands with two times the loading capacity.

Invitrogen Novex Tris-Glycine Mini Gels, WedgeWell format, are polyacrylamide gels that provide reproducible separation of a wide range of proteins into well-resolved bands with two times the loading capacity. Novex Tris-Glycine Mini Gels are based on traditional Laemmli protein electrophoresis that allow the use of Laemmli sample and running buffers.

Features of Novex Tris-Glycine gels, WedgeWell format, include:

• Improved shelf life—store gels for up to 7 months at 4°C
• Diversity—use for native and denaturing protein assays
• Wedge-shaped wells—easily load up to two times more sample volume
• Fast run conditions—quickly separate your proteins using constant voltage in less than 60 minutes

Choose the right gel format for your experiments

Novex Tris-Glycine gels, WedgeWell format, come in a variety of fixed concentrations from 6% to 18%, as well as gradients with ranges of 4–12%, 4–20%, 8–16%, and 10–20%. You can select from our many well formats, including 10-, 12-, and 15-well, and different pack sizes.

Run your proteins in native or denatured form

Novex Tris-Glycine gels, WedgeWell format, do not contain SDS and can be used to run your proteins in native or in denatured form. For denatured proteins, we recommend using Novex Tris-Glycine SDS Sample Buffer (LC2676) and Novex Tris-Glycine SDS Running Buffer (LC26755). For native proteins, we recommend using Novex Tris-Glycine Native Sample Buffer (LC2673) and Novex Tris-Glycine Native Running Buffer (LC2672).

Convenient to use

Novex Tris-Glycine gels, WedgeWell format, have innovative wedge-shaped wells that allow up to two times more sample load volume than other 1.0 mm-thick gels. The wedge-shaped wells also have larger openings, making sample loading easier.

For transferring your proteins to a membrane, we recommend using Novex Tris-Glycine Transfer Buffer (LC3675) when preforming traditional wet transfer using the Invitrogen Mini Blot Module (B1000). Transfers can also be performed with rapid semi-dry transfer using the Invitrogen Power Blotter or rapid dry transfer using the iBlot 2 Gel Transfer Device (IB21001).

Specifications

• Color: Clear
• Gel Percentage: 4 to 20%
• Gel Size: Mini
• Gel Thickness: 1.0 mm
• Gel Type: Tris-Glycine Plus
• Sample Loading Volume: Up to 60 μL
• Separation of: Protein
• Separation Range: 20 to 200 kDa
• Separation Type: Denaturing
• Wells: 10-well
• Mode of Separation: Molecular Weight
• Quantity: 30 Gels (3 Boxes)
• Shipping Condition: Room Temperature
• Storage Requirements: Store at 4°C to 25°C. Do not freeze.
• Length (Metric): 8 cm
• Width (Metric): 8 cm
• Product Line: Novex, WedgeWell
• Product Type: Protein Gel
• For Use With (Equipment): XCell SureLock Mini-Cell

Frequently Asked Questions (FAQs)

Is it okay to use protein gels past their expiration date?

We do not recommend using gels past their expiration date because over time, the polyacrylamide hydrolyzes to acrylic acid and ammonia and this will affect the resolution of the proteins. Breakdown of polyacrylamide matrix is identified by:
- Ghost bands and doublets, seen first in the high molecular weight proteins
- Smiling of dye front across the gel, with bands in outer lanes becoming very slanted - proteins run slower there due to change in pH and pore size over time.

Can I run your protein gels overnight?

This is not really recommended, but it is always possible to increase run time by lowering the voltage of the run. In general, the relationships are linear - i.e., decreasing voltage by half will double the run time.

Do I need to increase the voltage when I run a 1.5 mm protein gel versus a 1.0 mm gel?

If you are running the gel at constant voltage, you do not need to increase the voltage regardless of the thickness of the gel.

Why do you recommend running your protein gels at constant voltage?

Using constant voltage allows the current and power to decrease during the run, providing a safety margin in case of a break in the system. Having lower power is a safety benefit and will also decrease the chances of experiencing an overheating of the gel. Further, the constant voltage setting does not need adjustment to account for differences in number or thickness of gels being run.