Invitrogen™ WT1 Recombinant Rabbit Monoclonal Antibody (6L9X6)

Rabbit Recombinant Monoclonal Antibody

Supplier:  Invitrogen™ MA542786

Catalog No. PIMA542786

Description

Positive test controls include: Rat testis. The target is usually found in the following locations: Cytoplasm, Nucleus, Nucleus speckle, nucleolus, nucleoplasm. Immunogen sequence: PPPPPPPPPH SFIKQEPSWG GAEPHEEQCL SAFTVHFSGQ FTGTAGACRY GPFGPPPPSQ ASSGQARMFP NAPYLPSCLE SQPAIRNQGY STVTFDGTPS YGHTPSHHAA QFPNHSFKHE DPMGQQGSLG EQQYSVPPPV YGCHTPTDSC TGSQALLLRT PYSSDNLYQM TSQLECMTWN QMNLGATLKG V.

The Wilms Tumor 1 gene encodes one of the WPP domain-interacting proteins (Wilms Tumor 1/AT5G11390, WIT2/AT1G68910) required for RanGAP nuclear envelope association in root tip cells. Ran GTPase plays essential roles in multiple cellular processes, including nucleocytoplasmic transport, spindle formation, and postmitotic nuclear envelope reassembly. The cytoplasmic Ran GTPase activating protein RanGAP is critical to establish a functional RanGTP/RanGDP gradient across the nuclear envelope and is associated with the outer surface of the nuclear envelope in metazoan and higher plant cells. Arabidopsis thaliana RanGAP association with the root tip nuclear envelope requires a family of likely plant-specific nucleoporins combining coiled-coil and transmembrane domains (CC-TMD) and WPP domain-interacting proteins (WIPs). Wilms Tumor 1 and WIT2 have been identified as a second family of CC-TMD proteins, structurally similar, yet clearly distinct from the WIP family, that is required for RanGAP nuclear envelop association in root tip cells.

Specifications

• Antigen: WT1
• Classification: Recombinant Monoclonal
• Concentration: 0.6 mg/mL
• Formulation: PBS with 0.05% BSA, 50% glycerol and 0.02% sodium azide; pH 7.3
• Gene Accession No.: P19544, P22561, P49952
• Gene Symbols: WT1
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 60-250 of human WT1 (P19544).
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat
• Product Type: Antibody
• Isotype: IgG

• Applications: ELISA, Immunohistochemistry (Paraffin), Western Blot
• Clone: 6L9X6
• Conjugate: Unconjugated
• Gene: WT1
• Gene Alias: AWT1; D630046I19Rik; EWS-WT1; GUD; NPHS4; WAGR; Wilms tumor 1; Wilms tumor 1 homolog; Wilms tumor protein; Wilms tumor protein homolog; Wilms tumor protein isoform Ex4a(+); WIT1; WIT-1; WIT-2; WT; Wt1; Wt-1; WT1 antisense RNA; WT1AS; WT1-AS; WT1-AS1; WT33
• Host Species: Rabbit
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 22431, 24883, 7490
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Form: Liquid