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Invitrogen™ Z'-LYTE™ Kinase Assay Kit - Ser/Thr 16 Peptide

Catalog No. PV3802
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800 x 20 μL Assays
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PV3802 800 x 20 μL Assays
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Catalog No. PV3802 Supplier Invitrogen™ Supplier No. PV3802
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Description

Z'-LYTE™ Kinase Assay Kit - Ser/Thr 16 Peptide

A coumarin and fluorescein-labeled peptide substrate which can be used for kinase screening in an antibody-free format. This peptide is based upon CDC25C, containing Ser-198.
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Specifications

Product Line Z´-LYTE
Target Serine/Threonine Kinases
For Use With (Application) FRET
Assay Serine/Threonine Kinase Assay
Content And Storage Z´-LYTE™ Kinase Assay Kits contain all necessary reagents for performing primary or secondary screens. Most kit components should be stored at -20°C, and some kit components should be stored at -80°C. See COA for specific storage instructions.
Assay Entry Biochemical phospho-specific activity (no antibody)
Label or Dye Methylcoumarin
Product Type Kinase Assay Kit
Quantity 800 x 20 μL Assays
Format 384-well plate
Sample Type CDC25C residues 193-204, centered at Ser-198 (ELMEFSLKDQEA)
Excitation/Emission 405⁄465, 535
No. of Reactions 800 Reactions
References JBC 278:25277 (2003)
Readout End Point
Shipping Condition Dry Ice
Substrate Peptide-Based Substrate
Target Entry GRK7, ADRBK1, ADRBK2, ACVR1B, PLK2, PLK3, GRK6, GRK5, GRK4, PLK1
Validated Kinases ACVR1B, ADRBK1, ADRBK2, GRK4, GRK5, GRK6, GRK7, PLK1, PLK2, PLK3
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For Research Use Only. Not for use in diagnostic procedures.

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