Invitrogen™ Zap-70 Monoclonal Antibody (1E7.2)

Mouse Monoclonal Antibody

Supplier:  Invitrogen™ 376500

Catalog No. 37-650-0

Description

Zap-70 Monoclonal Antibody for Western Blot, Flow, IP

ZAP-70 is a cytosolic protein tyrosine kinase that is a member of the Syk family of proteins. ZAP-70 is expressed exclusively in T-cells and natural killer cells and is required for T-cell receptor activation. Upon T cell antigen receptor (TCR) engagement, ZAP70 is phosphorylated on tyrosines 292, 315 and 319 in the interdomain B, located between the SH2 and kinase domains. Phosphorylation of both tyrosines 315 (a Vav binding site) and 319 (a Lck binding site) enhances ZAP70 function in mediating lymphocyte signaling, while tyrosine 292 terminates the transient activation of ZAP70 and attentuates lymphocyte signaling.

Specifications

• Antigen: Zap-70
• Classification: Monoclonal
• Concentration: 0.5 mg/mL
• Formulation: PBS with 0.1% sodium azide; pH 7.4
• Gene Accession No.: P43403, P43404
• Gene Symbols: ZAP70
• Immunogen: Synthetic peptide derived from the region between the second SH2 domain and the kinase domain of human ZAP-70.
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Human, Mouse
• Product Type: Antibody
• Isotype: IgG1

• Applications: Flow Cytometry, Immunoprecipitation, Western Blot
• Clone: 1E7.2
• Conjugate: Unconjugated
• Gene: ZAP70
• Gene Alias: 70 kDa zeta-associated protein; 70 kDa zeta-chain associated protein; ADMIO2; EC 2.7.10.2; FLJ17670; FLJ17679; I79_004087; IMD48; kinase ZAP70; mrtle; mur; Selective T cell defect; Srk; STCD; STD; syk-related protein tyrosine kinase; syk-related tyrosine kinase; Tyrosine-protein kinase ZAP-70; Tyrosine-protein kinase ZAP-70;TZK; TZK; ZA70; Zap70; ZAP-70; Zeta chain associated protein kinase 70kDa; zeta chain of T cell receptor associated protein kinase; zeta chain of T cell receptor associated protein kinase 70; zeta chain of T cell receptor associated protein kinase 70kDa; zeta-chain (TCR) associated protein kinase; zeta-chain (TCR) associated protein kinase 70; zeta-chain (TCR) associated protein kinase 70kDa; zeta-chain associated protein kinase 70kDa; zeta-chain associated protein kinase, 70kD
• Host Species: Mouse
• Purification Method: Protein A
• Regulatory Status: RUO
• Gene ID (Entrez): 22637, 7535
• Content And Storage: -20°C
• Form: Liquid

Safety and Handling

WARNING: Reproductive Harm - www.P65Warnings.ca.gov