Invitrogen™ ZNRF3 Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA5143891

Catalog No. PIPA5143891

Description

Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL. Positive Control - WB: human CACO-2 whole cell, human A549 whole cell, human Hacat whole cell. ICC/IF: U20S cell. Flow: PC-3 cell. Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

The ZNRF3 gene encodes a transmembrane E3 ubiquitin ligase that plays a critical role in regulating the Wnt signaling pathway, which is essential for various biological processes, including embryonic development and cell proliferation. ZNRF3 functions by negatively regulating this pathway through ubiquitination and degradation of Frizzled receptors, thereby suppressing the Wnt signaling. Located on chromosome 22, ZNRF3 acts as a tumor suppressor gene by inhibiting oncogenic Wnt signaling and has been implicated in the formation and progression of several cancers, particularly colorectal cancer. Moreover, ZNRF3 interacts with R-spondin proteins which are known to augment Wnt signaling by targeting ZNRF3 for degradation, thus facilitating cell differentiation and proliferation. Alterations in ZNRF3 expression or mutations can lead to aberrant Wnt signaling, contributing to tumorigenesis. Additionally, ZNRF3's role extends to its participation in lipid metabolism and maintenance of bone homeostasis. It has been studied as a potential therapeutic target due to its central involvement in these regulatory pathways.

Specifications

• Antigen: ZNRF3
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: ZNRF3
• Gene Alias: BK747E2.3; CTA-292E10.6; E3 ubiquitin-protein ligase ZNRF3; Gm1167; KIAA1133; novel C3HC4 type Zinc finger (ring finger); rCG35969-like; RING finger protein 203; RING-type E3 ubiquitin transferase ZNRF3; RNF203; similar to ring finger protein 43; zinc and ring finger 3; zinc/RING finger protein 3; ZNRF3
• Host Species: Rabbit
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 84133
• Content And Storage: -20°C
• Form: Lyophilized

• Applications: ELISA, Flow Cytometry, Western Blot, Immunocytochemistry
• Concentration: 500 μg/mL
• Formulation: PBS with 4mg trehalose and no preservative
• Gene Accession No.: Q9ULT6
• Gene Symbols: ZNRF3
• Immunogen: E.coli-derived human ZNRF3 recombinant protein (Position: R54-E913).
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG