Invitrogen™ ZO-1 Monoclonal Antibody (ZO1-1A12), FITC

Mouse Monoclonal Antibody

Supplier:  Invitrogen™ 339111

Catalog No. 33-911-1

Description

Species reactivity includes human (Caco-2 Cell line) and dog (MDCK cell line). Based on sequence homology, reactivity with other species is likely but has not been confirmed. This monoclonal antibody detects ZO-1alpha+ and ZO-1alpha- isoforms. The antibody is specific for ZO-1 proteins; cross-reactivity with the related ZO-2 protein has not been observed. For this FITC conjugate the excitation (EX)/emission (EM) are 494/520 (nm).

ZO-1 forms complexes with either ZO-2 or ZO-3. In addition, these proteins can also associate with claudin, occludin and F-actin, at tight junction stands, where they provide a linkage between the actin cytoskeleton and the tight junction. ZO-1 expression is significantly reduced in many breast cancer lines. ZO-2 and ZO-3 are ubiquitously expressed within epithelial tight junctions, and unlike ZO-1, which is also expressed at cell junctions of cardiac myocytes, ZO-2 is not expressed in nonepithelial tissue. ZO-1 is a protein located on a cytoplasmic membrane surface of intercellular tight junctions. ZO-1 may be involved in signal transduction at cell-cell junctions. Two transcript variants encoding distinct isoforms have been identified for the ZO-1 gene. Diseases associated with ZO-1 dysfunction include Celiac Disease and Congenital Nephrotic Syndrome.

Specifications

• Antigen: ZO-1
• Classification: Monoclonal
• Concentration: 0.5 mg/mL
• Formulation: PBS with 1% BSA, 50% glycerol and 0.1% sodium azide; pH 7.4
• Gene Accession No.: O97758, Q07157
• Gene Symbols: TJP1
• Immunogen: Human recombinant ZO-1 fusion protein encompassing amino acids 334-634.
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Human, Canine
• Product Type: Antibody
• Isotype: IgG1 κ

• Applications: ELISA, Western Blot, Immunocytochemistry
• Clone: ZO1-1A12
• Conjugate: FITC
• Gene: TJP1
• Gene Alias: DKFZp686M05161; I79_005485; MGC133289; OTTHUMP00000174520; Tight junction protein 1; tight junction protein 1 (zona occludens 1); tight junction protein ZO-1; Tjp1; zo1; ZO-1; ZO-1 MDCK; zona occludens 1; zona occludens protein 1; zonula occludens 1; zonula occludens 1 protein; zonula occludens protein 1
• Host Species: Mouse
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 403752, 7082
• Content And Storage: 4°C, store in dark
• Form: Liquid