Invitrogen™ ZO-1 Polyclonal Antibody

Catalog No.	Quantity
PIPA585256	100 μL

Catalog No. PIPA585256 Supplier Invitrogen™ Supplier No. PA585256

Description

Rabbit Polyclonal Antibody

Keep as concentrated solution. Predicted reactivity: Mouse (95%), Rat (94%), Dog (95%). Positive Control: 293T, A431, HeLa, HepG2, A549, H1299, HCT116, mouse testis. Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

ZO-1 forms complexes with either ZO-2 or ZO-3. In addition, these proteins can also associate with claudin, occludin and F-actin, at tight junction stands, where they provide a linkage between the actin cytoskeleton and the tight junction. ZO-1 expression is significantly reduced in many breast cancer lines. ZO-2 and ZO-3 are ubiquitously expressed within epithelial tight junctions, and unlike ZO-1, which is also expressed at cell junctions of cardiac myocytes, ZO-2 is not expressed in nonepithelial tissue. ZO-1 is a protein located on a cytoplasmic membrane surface of intercellular tight junctions. ZO-1 may be involved in signal transduction at cell-cell junctions. Two transcript variants encoding distinct isoforms have been identified for the ZO-1 gene. Diseases associated with ZO-1 dysfunction include Celiac Disease and Congenital Nephrotic Syndrome.

Specifications

• Antigen: ZO-1
• Applications: Immunohistochemistry (Frozen), Immunoprecipitation, Western Blot, Immunocytochemistry, Western Blot
• Classification: Polyclonal
• Concentration: 0.58 mg/mL
• Conjugate: Unconjugated
• Formulation: PBS with 20% glycerol and 0.025% ProClin 300; pH 7
• Gene: TJP1
• Gene Accession No.: P39447, Q07157
• Gene Alias: DKFZp686M05161; I79_005485; MGC133289; OTTHUMP00000174520; Tight junction protein 1; tight junction protein 1 (zona occludens 1); tight junction protein ZO-1; Tjp1; zo1; ZO-1; ZO-1 MDCK; zona occludens 1; zona occludens protein 1; zonula occludens 1; zonula occludens 1 protein; zonula occludens protein 1
• Gene Symbols: TJP1
• Host Species: Rabbit
• Immunogen: Recombinant protein encompassing a sequence within the N-terminus region of human ZO-1. The exact sequence is proprietary.
• Purification Method: Antigen affinity chromatography
• Quantity: 100 μL
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 21872, 7082
• Target Species: Human, Mouse
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Product Type: Antibody
• Form: Liquid
• Isotype: IgG