Invitrogen™ ZO-3 Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ 364000

Catalog No. 36-400-0

Description

36-4000 was used successfully in the western blot analysis of ZO-3 in MDCK cell lysate.

ZO-3 is a member of the membrane associated guanylate kinse (MAGUK) family and are considered tight junction-associated proteins. Tight junctions are complexes of proteins that create intercellular boundaries between the plasma membrane domains of epithelial and endothelial cells. ZO-3 is believed to have both structural and signaling roles, and is characteristically defined by three protein-protein interaction modules: the PDZ domain, the SH3 domain and the guanylate kinase (GuK) domain. ZO-1 forms complexes with either ZO-2 or ZO-3. In addition, these proteins can also associate with claudin, occludin and F-actin at tight junction stands, where they provide a linkage between the actin cytoskeleton and the tight junction. ZO-1 expression is significantly reduced in many breast cancer lines. ZO-2 and ZO-3 are ubiquitously expressed within epithelial tight junctions, and unlike ZO-1, which is also expressed at cell junctions of cardiac myocytes, ZO-2 is not expressed in nonepithelial tissue.

Specifications

• Antigen: ZO-3
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: TJP3
• Gene Alias: tight junction protein 3; tight junction protein 3 (zona occludens 3); tight junction protein ZO-3; TJP3; TJP-3; ZO3; ZO-3; Zona occludens protein 3; zonula occludens protein 3
• Host Species: Rabbit
• Purification Method: Antigen Affinity Chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 27134, 27375, 314640, 403852
• Content And Storage: -20°C
• Form: Liquid

• Applications: ELISA, Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot, Immunocytochemistry
• Concentration: 0.25 mg/mL
• Formulation: PBS with 0.1% sodium azide; pH 7.4
• Gene Accession No.: O62683, O95049, Q9QXY1
• Gene Symbols: TJP3
• Immunogen: Synthetic peptide derived from an internal region of the human ZO-3 protein.
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat, Canine
• Product Type: Antibody
• Isotype: IgG