Zymo Research Corporation ZymoBIOMICS Gut Microbiome Standard, 10 preps

Supplier:  Zymo Research Corporation D6331

The standard presents multiple challenges for NGS pipelines, such as tough-to-lyse Gram-positive bacteria (e.g. Roseburia hominis) to test lysis efficiency, genomes with a wide range of GC content to test sequencing coverage bias, lowabundance pathogenic organisms for detection limit assessment and 5 different strains of E. coli to test taxonomic resolution. These challenge points can be used to expose artifacts, errors, and bias in microbiomics or metagenomics workflows. Serving as a defined input, this standard can be used to guide construction and optimization of entire workflows or as a quality control tool for inter-lab studies.The microbial standard is accurately characterized and contains negligible impurity (< 0.01%). It was constructed by pooling cells from pure cultures of 21 microbial strains. The cells from each pure culture were quantified before pooling. After mixing, the microbial composition was confirmed using NGS-based sequencing.

Catalog No. NC1843317