StemgentSupplier Diversity Partner Y27632 WEIGHT 2NG DFS Item

NC9818743 Y27632 is a cell-permeable small molecule Rho-associated kinase (ROCK) inhibitor. Size 2 mg. Y27632 has been found to prevent apoptosis as well as enhance the survival and cloning efficiency of dissociated human embryonic stem (ES) cells without affecting their self-renewal properties or pluripotency. This molecule has also been shown to enhance survival during the transplantation of ES cell-derived neural precursors. Y27632 in combination with Pifithrin-? significantly improves cell recovery after cryopreservation. Y27632 is a cell-permeable small molecule Rho-associated kinase (ROCK) inhibitor. Size 2 mg. Y27632 has been found to prevent apoptosis as well as enhance the survival and cloning efficiency of dissociated human embryonic stem (ES) cells without affecting their self-renewal properties or pluripotency. This molecule has also been shown to enhance survival during the transplantation of ES cell-derived neural precursors. Y27632 in combination with Pifithrin-? significantly improves cell recovery after cryopreservation.

StemgentSupplier Diversity Partner CHIR99021 IN SOL 2MG-10MM DFS Item

NC0391842 The aminopyrimidine CHIR99021 is the most selective inhibitor of glycogen synthase kinase 3i� (GSK-3i�) reported to date1,2. Unlike other potent inhibitors of GSK-3, CHIR99201 does not exhibit cross-reactivity against cyclin-dependent kinases (CDKs) and shows a 350-fold selectivity toward GSK-3i� compared to CDKs3. along with the elimination of differentiation-inducing signaling from mitogen-activated protein kinases, using CHIR99021 to block the activity of GSK-3i� enables the self-renewal of embryonic stem cells4. Stemoleculea, c CHIR99021 in Solution is a ready to use 10 mM stock solution for stem cell culture. The aminopyrimidine CHIR99021 is the most selective inhibitor of glycogen synthase kinase 3i� (GSK-3i�) reported to date1,2. Unlike other potent inhibitors of GSK-3, CHIR99201 does not exhibit cross-reactivity against cyclin-dependent kinases (CDKs) and shows a 350-fold selectivity toward GSK-3i� compared to CDKs3. along with the elimination of differentiation-inducing signaling from mitogen-activated protein kinases, using CHIR99021 to block the activity of GSK-3i� enables the self-renewal of embryonic stem cells4. Stemoleculea, c CHIR99021 in Solution is a ready to use 10 mM stock solution for stem cell culture.

StemgentSupplier Diversity Partner LDN-193189 FREE SALT 2MG DFS Item

NC0054000 Stemoleculea, c LDNaE�193189 Stemoleculea, c LDNaE�193189

StemgentSupplier Diversity Partner STEMOLECULE CHIR99021 (2MG) DFS Item

NC9785126 6- [[2- [[4- (2, 4- dichlorophenyl)- 5- (5- methyl- 1H- imidazol- 2- yl)- 2 pyrimidinyl]amino]ethyl]amino]- 3- pyridinecarbonitrile. 252917-06-9. 2mg. 6- [[2- [[4- (2, 4- dichlorophenyl)- 5- (5- methyl- 1H- imidazol- 2- yl)- 2 pyrimidinyl]amino]ethyl]amino]- 3- pyridinecarbonitrile. 252917-06-9. 2mg.

StemgentSupplier Diversity Partner STEMFECT RNA TRANSFECTION KIT DFS Item

NC0146453 Stemfect™ Rna Transfection Kit Set Contents: Stemfect Rna Transfection Reagent: 750 Μl Stemfect Transfection Buffer: 30Ml The Stemfect Rna Transfection Kit Is A Proprietary Mixture Of Lipid Components Is Specifically Designed For In Vitro Rna Transfection. This Kit Has Been Shown To Deliver Mrna, Sirna And Mirna. Stemfect Rna Transfection Kit Provides Greater Than Or Equal To 90% Transfection Efficiency Of Mrna Into A Range Of Cell Types With Greater Than 95% Viability; These Cell Types Include Human Embryonic Stem (Hes) Cells, Jurkat Cells, Human Fibroblasts, And Hek293T Cells. Stemfect Rna Transfection Kit Has Been Successfully Used To Deliver Rna To Human Es Cells, Dendritic Cells, And Retinal Pigment Epithelial Cells. There Is No Need To Serum-Starve Cells Using This Kit As The Transfection Efficiency Remains Greater Than Or Equal To 90% In The Presence Of Serum. Stemfect™ Rna Transfection Kit Set Contents: Stemfect Rna Transfection Reagent: 750 Μl Stemfect Transfection Buffer: 30Ml The Stemfect Rna Transfection Kit Is A Proprietary Mixture Of Lipid Components Is Specifically Designed For In Vitro Rna Transfection. This Kit Has Been Shown To Deliver Mrna, Sirna And Mirna. Stemfect Rna Transfection Kit Provides Greater Than Or Equal To 90% Transfection Efficiency Of Mrna Into A Range Of Cell Types With Greater Than 95% Viability; These Cell Types Include Human Embryonic Stem (Hes) Cells, Jurkat Cells, Human Fibroblasts, And Hek293T Cells. Stemfect Rna Transfection Kit Has Been Successfully Used To Deliver Rna To Human Es Cells, Dendritic Cells, And Retinal Pigment Epithelial Cells. There Is No Need To Serum-Starve Cells Using This Kit As The Transfection Efficiency Remains Greater Than Or Equal To 90% In The Presence Of Serum.

StemgentSupplier Diversity Partner CRYOSTEM FREEZING MEDIUM 50ML DFS Item

NC0507468 CryoStem Freezing Medium (50ml) is a ready-to-use solution for the xeno-free cryopreservation of human embryonic stem (ES), induced pluripotent stem (iPS) and mesenchymal stem cells. Pluripotent stem cells preserved with CryoStem Freezing Medium result in high cell viability and recovery and express typical pluripotent markers after thawing. CryoStem Freezing Medium was developed to maintain xeno-free conditions during cryopreservation when culturing cells in a xeno-free environment. CryoStem Freezing Medium has been tested on human ES cells (H1, H9 and HuES9) and mesenchymal stem cells. Superior results were obtained in comparison with both serum-containing freezing media as well as competing serum-free products, making this an ideal product for cryopreservation of valuable human stem cell cultures. CryoStem Freezing Medium (50ml) is a ready-to-use solution for the xeno-free cryopreservation of human embryonic stem (ES), induced pluripotent stem (iPS) and mesenchymal stem cells. Pluripotent stem cells preserved with CryoStem Freezing Medium result in high cell viability and recovery and express typical pluripotent markers after thawing. CryoStem Freezing Medium was developed to maintain xeno-free conditions during cryopreservation when culturing cells in a xeno-free environment. CryoStem Freezing Medium has been tested on human ES cells (H1, H9 and HuES9) and mesenchymal stem cells. Superior results were obtained in comparison with both serum-containing freezing media as well as competing serum-free products, making this an ideal product for cryopreservation of valuable human stem cell cultures.

StemgentSupplier Diversity Partner STEMOLECULE WNT INHB IWP-2 2MG DFS Item

NC9086357 Stemolecule™ Wnt Inhibitor IWP-2. Stemolecule Wnt Inhibitor IWP-2 was identified in a high throughput screen for antagonists of the Wnt/β-catenin pathway. Wnt Inhibitor IWP-2 prevents palmitylation of Wnt proteins by Porcupine (Porcn), a membrane-bound O-acyltransferase, thereby blocking Wnt secretion and activity. It also blocks phosphorylation of the Lrp6 receptor and accumulation of both Dvl2 and β-catenin1. Stemolecule™ Wnt Inhibitor IWP-2. Stemolecule Wnt Inhibitor IWP-2 was identified in a high throughput screen for antagonists of the Wnt/β-catenin pathway. Wnt Inhibitor IWP-2 prevents palmitylation of Wnt proteins by Porcupine (Porcn), a membrane-bound O-acyltransferase, thereby blocking Wnt secretion and activity. It also blocks phosphorylation of the Lrp6 receptor and accumulation of both Dvl2 and β-catenin1.

StemgentSupplier Diversity Partner STEMOLECULE PURMORPHAMINE 5MG DFS Item

NC9988977 Purmorphamine is a small molecule that promotes the differentiation of human and murine mesenchymal progenitor cells into osteoblasts. The mechanism of action study of Purmorphamine indicates that it is an agonist of Smoothened, a 7-transmembrane receptor of the hedgehog signaling pathway. Purmorphamine can also be used to replace sonic hedgehog for the generation of motor neurons from human embryonic stem cells. Size: 5mg. Molecular weight: 520.6. Purity: Greater than 96% by HPLC analysis. For a 10 mM concentrated stock solution of Purmorphamine, reconstitute the compound by adding 960.4 ul of DMSO to the entire contents of the vial. If precipitate is observed, warm the solution to 37C for 2 to 5 minutes. For use in cell culture, warm the medium just prior to adding the reconstituted compound. Once the compound is added, mix and filter-sterilize the medium using a 0.2 uM low-protein binding filter. Purmorphamine is soluble in DMSO at 50 mM. 483367-10-8 Purmorphamine is a small molecule that promotes the differentiation of human and murine mesenchymal progenitor cells into osteoblasts. The mechanism of action study of Purmorphamine indicates that it is an agonist of Smoothened, a 7-transmembrane receptor of the hedgehog signaling pathway. Purmorphamine can also be used to replace sonic hedgehog for the generation of motor neurons from human embryonic stem cells. Size: 5mg. Molecular weight: 520.6. Purity: Greater than 96% by HPLC analysis. For a 10 mM concentrated stock solution of Purmorphamine, reconstitute the compound by adding 960.4 ul of DMSO to the entire contents of the vial. If precipitate is observed, warm the solution to 37C for 2 to 5 minutes. For use in cell culture, warm the medium just prior to adding the reconstituted compound. Once the compound is added, mix and filter-sterilize the medium using a 0.2 uM low-protein binding filter. Purmorphamine is soluble in DMSO at 50 mM. 483367-10-8

StemgentSupplier Diversity Partner STEMOLECULE DORSOMORPHIN 2MG DFS Item

NC0275327 Stemolecule Dorsomorphin dihydrochloride (also known as Compound C) is a potent inhibitor of AMP-activated protein kinase (AMPK) (Ki=109 nM) and bone morphogenic protein (BMP) signaling. It was identified in a screen for compounds that perturb dorsoventral axis formation in zebrafish3. Dorsomorphin functions through inhibition of BMP type I receptors ALK2, ALK3 and ALK6 and thus blocks BMP-mediated SMAD1/5/8 phosphorylation. BMP signaling coordinates developmental patterning and have essential physiological roles in mature organisms. Dorsomorphin has been used to probe BMP signaling in iron-hepcidin homeostasis, cardiomyogenesis and osteogenesis. Stemolecule Dorsomorphin dihydrochloride (also known as Compound C) is a potent inhibitor of AMP-activated protein kinase (AMPK) (Ki=109 nM) and bone morphogenic protein (BMP) signaling. It was identified in a screen for compounds that perturb dorsoventral axis formation in zebrafish3. Dorsomorphin functions through inhibition of BMP type I receptors ALK2, ALK3 and ALK6 and thus blocks BMP-mediated SMAD1/5/8 phosphorylation. BMP signaling coordinates developmental patterning and have essential physiological roles in mature organisms. Dorsomorphin has been used to probe BMP signaling in iron-hepcidin homeostasis, cardiomyogenesis and osteogenesis.

StemgentSupplier Diversity Partner ALKALINE PHOSPHATE STAINING KI DFS Item

NC0088922 Specifications: 50 assays. Kit ComponentsFix Solution: 25 ml. AP Staining Solution A: 10 ml.AP Staining Solution B: 10 ml.AP Staining Solution C: 10 ml Specifications: 50 assays. Kit ComponentsFix Solution: 25 ml. AP Staining Solution A: 10 ml.AP Staining Solution B: 10 ml.AP Staining Solution C: 10 ml

StemgentSupplier Diversity Partner NUTRISTEM XF/FF CULTURE MEDIUM DFS Item

NC0345861 NutriStem XF/FF Culture Medium for Human iPS and ES Cells, 500ml. NutriStem XF/FF Culture Medium is a fully defined xeno-free, low growth factor human embryonic stem (ES) and induced pluripotent stem (iPS) cell feeder-free culture medium that enables maintenance and expansion of pluripotent stem cells. NutriStem Medium can be used to culture pluripotent stem cells for at least 20 passages while retaining pluripotency marker expression, robust proliferation with a normal karyotype, and the ability to differentiate into cells of all three germ layers in vitro and in vivo. NutriStem Medium offers the ability to culture cells in a completely xeno-free medium without the need for high levels of basic FGF and other stimulatory growth factors and cytokines. In addition, the superior cell attachment and proliferation observed with NutriStem Medium aid high-throughput screening applications. NutriStem XF/FF Culture Medium for Human iPS and ES Cells, 500ml. NutriStem XF/FF Culture Medium is a fully defined xeno-free, low growth factor human embryonic stem (ES) and induced pluripotent stem (iPS) cell feeder-free culture medium that enables maintenance and expansion of pluripotent stem cells. NutriStem Medium can be used to culture pluripotent stem cells for at least 20 passages while retaining pluripotency marker expression, robust proliferation with a normal karyotype, and the ability to differentiate into cells of all three germ layers in vitro and in vivo. NutriStem Medium offers the ability to culture cells in a completely xeno-free medium without the need for high levels of basic FGF and other stimulatory growth factors and cytokines. In addition, the superior cell attachment and proliferation observed with NutriStem Medium aid high-throughput screening applications.

StemgentSupplier Diversity Partner STEMFACTOR BMP-4 HUMAN RECOMB DFS Item

NC0368818 Stemfactor, BMP-4, Human Recombinant, 10g Bone Morphogenetic Protein 4 (BMP-4) is a polypeptide belonging to the TGF-I protein super-family. BMP-4 is involved in bone and cartilage development; more specifically, in tooth and limb development fracture repair.In human embryonic development, BMP-4 is a critical signaling molecule req for the early differentiation of the embryo and establishment of a dorsal-ventral axis. BMP-4 plays an important role indifferentiation of overlying ectodermal tissue. Inhibition of the BMP-4 signal causes the ectoderm to differentiate into the neural plate. In cultured stem cells, BMP-4 plays a distinct role in mouse and human embryonic stem (ES) cells. BMP-4 supports LIF as a positive factor for mouse ES cell self-renewal. In contrast, BMP-4 induces extra-embryonic trophoblast differentiation in human ES cells. Stemfactor, BMP-4, Human Recombinant, 10g Bone Morphogenetic Protein 4 (BMP-4) is a polypeptide belonging to the TGF-I protein super-family. BMP-4 is involved in bone and cartilage development; more specifically, in tooth and limb development fracture repair.In human embryonic development, BMP-4 is a critical signaling molecule req for the early differentiation of the embryo and establishment of a dorsal-ventral axis. BMP-4 plays an important role indifferentiation of overlying ectodermal tissue. Inhibition of the BMP-4 signal causes the ectoderm to differentiate into the neural plate. In cultured stem cells, BMP-4 plays a distinct role in mouse and human embryonic stem (ES) cells. BMP-4 supports LIF as a positive factor for mouse ES cell self-renewal. In contrast, BMP-4 induces extra-embryonic trophoblast differentiation in human ES cells.

StemgentSupplier Diversity Partner STEMOLECULE WNT INHB IWP-4 2MG DFS Item

NC9086360 Stemolecule Wnt Inhibitor IWP-4, 2mg. Wnt Inhibitor IWP-4 was identified in a high throughput screen for antagonists of the Wnt / b-catenin pathway. Wnt Inhibitor IWP-4 prevents palmitylation of Wnt proteins by Porcupine (Porcn), a membrane-bound O-acyltransferase, there by blocking Wnt secretion and activity. It also blocks phosphorylation of the Lrp6 receptor and accumulation of both Dvl2 and b-catenin1. Chemical name: 2-(3,4,6,7-tetrahydro-3-(2-methoxyphenyl)-4-oxothieno[3,2-d]pyrimidin-2-ylthio)-N-(6-methylbenzo[d]thiazol-2-yl)acetamide 686772-17-8. Stemolecule Wnt Inhibitor IWP-4, 2mg. Wnt Inhibitor IWP-4 was identified in a high throughput screen for antagonists of the Wnt / b-catenin pathway. Wnt Inhibitor IWP-4 prevents palmitylation of Wnt proteins by Porcupine (Porcn), a membrane-bound O-acyltransferase, there by blocking Wnt secretion and activity. It also blocks phosphorylation of the Lrp6 receptor and accumulation of both Dvl2 and b-catenin1. Chemical name: 2-(3,4,6,7-tetrahydro-3-(2-methoxyphenyl)-4-oxothieno[3,2-d]pyrimidin-2-ylthio)-N-(6-methylbenzo[d]thiazol-2-yl)acetamide 686772-17-8.

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