Histology Reagents
Electron Microscopy Sciences Acid Alcohol 1% in 75% Alcohol 500 ML
Used in Gridley's Method for Endamoeba Histolytica. For bacteria stain: Amoebae, their nuclei and ingested erythrocytes.
Electron Microscopy Sciences Acetone-Xylene 500 ML
Used in Alizarin Red S for calcium deposits staining. See references: McGee-Russell, S.M., J. Histochem., 6:22-42, 1958 or Clark, G., (ed), Staining Procedures, 3rd d., Williams & Wilkins, Baltimore, p. 140, c. 1973.
Electron Microscopy Sciences Eosin Y Eosin B Solution 500 ML
Eosin Y solution is a prepared, ready-to-use, high quality staining solution that saves time and effort and produces consistent uniform results.
Electron Microscopy Sciences Mammalian Ringer Solution 500 ML
Solution made up of salts including sodium chloride, potassium chloride, calcium chloride hexahydrate, sodium bicarbonate, and magnesium chloride hexahydrate.
Electron Microscopy Sciences Durcupan Single Component B
Single Component B Durcupan, Water Soluble- a water soluble embedding medium for EM based on an aliphatic polyepoxide.
Electron Microscopy Sciences Formaldehyde, Reagent, A.C.S. (Formalin), 50-00-0, 450ml
Molecular Formula CH2O, Molecular Weight 30.026, ACS Grade
Electron Microscopy Sciences Histo-Clear II, 1Gal
Histo-Clear II is a mixture of Aliphatic Hydrocarbon and Distilled essential oils. It is an excellent non-toxic clearing agent that is replacing Xylene.
Visikol Inc Visikol HISTO-1 30mL
Laboratory Reagent, Histological Reagent, Tissue Clearing Reagent, Visikol HISTO-1, 30mL. A simple and rapid tissue clearing formula that can be used to image tissues in 3D without damaging the underlying cellular morphology. Designed to pair with immunolabeling and reversible so that 3D histology can be followed up with traditional 2D histology. The Visikol HISTO tissue clearing approach is comprised of two different reagents called Visikol HISTO-1 and Visikol HISTO-2 whereas Visikol HISTO-1 is used first and Visikol HISTO-2 is used second. However, for small tissues such as micro-tissues and thin brain slices (500 um), only Visikol HISTO-1 is required and tissues can be imaged in Visikol HISTO-1. Larger tissues must be placed in Visikol HISTO-2 to finish clearing and then these samples can be imaged in Visikol HISTO-2.
Ted Pella Inc Osmium Tetroxide, 4% aqueous, 10 x 2ml
Solid vaporizes by sublimation. Should be used only under the hood. Affects eyes. Use eye protection. EM Grade, glass ampoules in black paper. No sticky labels. Osmium purity >99.95%. Light sensitive.
Electron Microscopy Sciences Bismuth Carbonate, Basic, Purified 25 GR
(Bismuth Subcarbonate)
CAS #5892-10-4 Approx. (BiO)₂CO₃ F.W. 509.96
Used for staining synapses. Pfenninger, K., Sandri, C., Albert, K. and Eugster, C.H. (1969). Contribution of the problem of structural organization of the presynaptic area. Brain Res., 12:10
Electron Microscopy Sciences 20% Paraformaldehyde (formaldehyde) aqueous solution, 10x10 mL
Paraformaldehyde - methanol free solution. A more efficient and rapid penetrant fixative used in combination with Glutaraldehyde and Acrolein fixatives. 10 mL ampoules sealed under inert gas.
Electron Microscopy Sciences Bouin's Fixative 500 ML
Used in Masson's Trichrome for Connective Tissue for stain nuclei, agentaffen granules, collagen, cytoplasm, keratin, etc. References: Sheehan and Hrapchak: Theory and Practice of Histotechnology, St. Louis. The Moxby Company 1980, p. 190
Volu Sol Acid Alcohol Decolorizer, 16 oz / 500 mL, Volu-Sol™
Decolorizer, for use in staining Acid Fast Bacteria in the Kinyoun, Modified Kinyoun, or Ziehl-Neelsen staining protocols