Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Bioworld TT Buffer, Ready-Pack (10X), 2 L
Tris-Tricine BufferTris-Tricine (TT) Buffer has been developed for both non-denaturing and denaturing electrophoresis of proteins, and offers several advantages over more conventionally used buffers. TT Buffer allows for optimal resolution of low molecular weight proteins and elimantes the glycine front often found in gel utilizing TG-SDS and other such beffers. This improved running buffer allows for resolution of peptides as low as 5kD. TT buffer is also excellent for protein electrophoresis where resolved products will be transferred to a nylon membrane. Unlike glycine, tricine will not interfere with membrane protein sequencing. The 10X pack just requires the addition of distilled H2O to make 1.0 Liter of 10X liquid. A simple 10-fold dilution with water provides a 1X solution, that makes an excellent running buffer for most applications. Technical Information:Ultra PureGradeStorage: RTHazard: Irritant
Sigma Aldrich Fine Chemicals Biosciences Glycine Buffer Solution | 100ml
Glycine is used as a bulking agent buffers. Glycine at low concentrations prevents pH decrease in solutions. It also stabilizes a protein when present in an amorphous state.
Boston Bioproducts Inc HBS-EP+ Buffer (1X, pH 7.4) - 4x500ml
HBS-EP Plus Buffer (1X pH 7 4) - 4x500ml (For Research Use and Further Manufacturing Only)
ATHENA ES HBS w/Phenol Red, 500 ml
Hepes Buffered Saline is available with or without Phenol Red. It is an isotonic saline buffered with Hepes, for use in rinsing cell monolayers prior to cell dissociation using PETTM or washing harvested cells.
Sigma Aldrich Fine Chemicals Biosciences BIS-TRIS BioPerformance Certified, suitable for cell culture, suitable for insect cell culture, >=98.0% | 6976-37-0 | MFCD00002853 | 25G
BIS-TRIS BioPerformance Certified, suitable for cell culture, suitable for insect cell culture, >=98.0% | Purity: >=98.0% | Mol Wt: 209.24 | 6976-37-0 | MFCD00002853 | 25G
Quality Biological Inc TE pH 7.4, 1000 mL
TE is composed of buffering agent Tris, and chelating agent EDTA. ¿ EDTA prevents degradation of DNA and RNA by chelating magnesium (or other divalent metal ions). ¿ Together, this Tris-EDTA based solution solubilizes DNA or RNA, while protecting it from degradation. ¿ Applications: TE is useful for reconstituting nucleic acids, during DNA precipitation and other DNA applications, storing and diluting DNA and RNA, breaking protein-links in immuno-histochemistry procedures, and unmasking antigens and epitopes in formalin-fixed and paraffin-embedded tissue sections. ¿ Storage Conditions: 15¿C to 30¿C ¿ Shipping Condition: ambient temperature ¿ Sterile Filtered ¿ DNase, RNase and Protease Tested ¿ Product and/or packaging contains recycled/renewable content ¿ Product and/or packaging is recyclable or reduces waste ¿ Product and/or packaging is safer to human and environmental health ¿ ¿ Max Shelf Life: 36 months
Research Products International Corp TRIS, ACS Grade, 1 Kilogram
TRIS, also known as tris(hydroxymethyl)aminomethane, is a chemical compound widely used in life science as a buffer.
Kd Medical Inc TE 1X, PH 8.0, 500 ML
TE buffer is a commonly used buffer solution in molecular biology, especially in procedures involving DNA or RNA. One 500ml bottle.
![Research Products International Corp Tris Base Ultra Pure [Tris (Hydroxymethyl) Aminomethane], 100 Grams](https://assets.fishersci.com/TFS-Assets/CCG/product-images/VN00006095-T60040-100.0.JPG-250.jpg)
Research Products International Corp Tris Base Ultra Pure [Tris (Hydroxymethyl) Aminomethane], 100 Grams
Tris Base Ultra Pure is a commonly used reagent and buffer component. Tris Base, Tris(hydroxymethyl)aminomethane, is a primary amine that acts as a buffering agent to maintain a stable pH in experimental procedures.
It is particularly effective in the pH range of 7.2 to 9.0, making it suitable for applications requiring slightly alkaline to mildly acidic pH conditions. Tris Base Ultra Pure is highly purified to remove impurities and contaminants, ensuring the highest quality for laboratory use.
The ultra-pure form is essential for applications where the presence of impurities can negatively impact experimental results. Tris Base Ultra Pure is commonly used in various molecular biology techniques such as DNA and RNA extraction, protein purification, and enzymatic assays.
It is also utilized in the preparation of electrophoresis buffers and PCR reactions.
Electron Microscopy Sciences Tris (Hydroxymethyl) Aminomethane Free Base, Reagent 100 GR
CAS #77-86-1 C₄H₁₁NO₃ F.W. 121.14
Assay: >99.9%
White crystalline powder. A 40% (w/v) aqueous solution. Hydroxymethyl Aminomethane.
Grade: Reagent
Teknova 1M Tris-HCl, pH 9.5. 1000mL, Sterile.
Tris (tris(hydroxymethyl)aminomethane) is used as a component ofbuffer solutions, especially for solutions ofnucleic acids. The useful buffer range for tris (pH 7–9) is similar to the pH of most living organisms. This, and its low cost, make tris one of the most common buffers in the biology/biochemistry laboratory.
![Research Products International Corp MOPS, Sodium Salt [3-(N-Morpholino)-propanesulfonic acid sodium salt], 1 Kilogram](https://assets.fishersci.com/TFS-Assets/CCG/product-images/VN00006095-M92040-1000.0.JPG-250.jpg)
Research Products International Corp MOPS, Sodium Salt [3-(N-Morpholino)-propanesulfonic acid sodium salt], 1 Kilogram
MOPS Sodium Salt, also known as 3-(N-Morpholino) propanesulfonic acid sodium salt, is a chemical compound that plays a significant role in the life science marketplace. It is a zwitterionic buffer commonly used in biological and biochemical research and is primarily utilized as a buffering agent to maintain a stable pH in various experimental procedures.
It is particularly effective in the pH range of 6.5 to 7.9, making it suitable for applications requiring slightly acidic to neutral pH conditions. MOPS Sodium Salt is commonly used in protein purification, enzyme assays, nucleic acid research, and electrophoresis techniques where a specific pH range needs to be maintained. Its buffering capacity and stability within the desired pH range make it valuable in these applications.
Sigma Aldrich Fine Chemicals Biosciences mPAGER 4-20 Bis-Tris Preca
mPAGE 4-20% Bis-Tris Precast Gel 10x8 cmUnless otherwise stated in our catalog or other company documentation accompanying the product(s) our products are intended for research use only and are not to be used for any other purpose which includes but is not limited to unauthorized commercial uses in vitro diagnostic uses ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.